Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi, People's Republic of China.
College of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi, People's Republic of China.
J Agric Food Chem. 2021 Jan 20;69(2):668-675. doi: 10.1021/acs.jafc.0c06822. Epub 2021 Jan 5.
A chemical study on the fruiting bodies of cultivated edible mushroom resulted in 14 metabolites including three new hispolon congeners, named inonophenols A-B and one new lanostane triterpenoid, named inonoterpene A. These structures were identified by NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and electronic circular dichroism (ECD) data analysis. All metabolites were assessed for neurotrophic, anti-inflammatory, and antioxidative activities. Among them, inonophenols B and C were the most active in promoting PC-12 cell neurite outgrowth at a concentration of 10 M. The phenolic derivatives reduced NO generation by lipopolysaccharide (LPS)-induced BV-2 microglial cells by suppressing the expression of toll-like receptor-4 (TLR-4) and the nuclear factor-kappa-B (NF-κB) signaling pathway as well as the inflammatory mediators including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, the phenolics showed antioxidant effects in DPPH scavenging assay with the IC values of 9.82-21.43 M. These findings showed that may be a new source of neurotrophic and protective agents against neurodegenerative disorders.
对栽培食用菌子实体的化学研究产生了 14 种代谢产物，包括 3 种新的棘孢木酮同系物，命名为异诺酚 A-B，以及 1 种新的羊毛甾烷三萜，命名为异诺萜烯 A。这些结构通过核磁共振、高分辨率电喷雾电离质谱 (HRESIMS) 和电子圆二色性 (ECD) 数据分析进行了鉴定。所有代谢产物均评估了神经营养、抗炎和抗氧化活性。其中，异诺酚 B 和 C 在 10 μM 浓度下最能促进 PC-12 细胞突起生长。酚衍生物通过抑制 Toll 样受体-4 (TLR-4) 和核因子-kappa-B (NF-κB) 信号通路以及诱导型一氧化氮合酶 (iNOS) 和环氧化酶-2 (COX-2) 等炎症介质的表达，降低脂多糖 (LPS) 诱导的 BV-2 小胶质细胞中 NO 的生成。此外，这些酚类化合物在 DPPH 清除试验中表现出抗氧化作用，IC 值为 9.82-21.43 μM。这些发现表明，可能是神经营养和保护剂的新来源，可预防神经退行性疾病。
