Chen Huan, Wang Xi, Jia Huanhuan, Tao Yin, Zhou Hong, Wang Mingyuan, Wang Xin, Fang Xiaoling
Department of Obstetrics and Gynecology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, People's Republic of China.
Guangdong Laboratory Animals Monitoring Institute, Guangdong Key Laboratory of Laboratory Animals, Guangzhou, Guangdong 510663, People's Republic of China.
Onco Targets Ther. 2020 Dec 29;13:13275-13283. doi: 10.2147/OTT.S281533. eCollection 2020.
Globally, cervical cancer (CC) is the fourth most common cancer affecting women. Although effective screening reduces its incidence, it remains one of the most serious cancers threatening the health of women. Therefore, the purpose of this study is to find new genes that can be used as potential biomarkers for the prognosis of CC.
After downloading three datasets such as GSE6791, GSE63678, and GSE63514 from the Gene Expression Omnibus (GEO), we combined the expression matrixes and analyzed them to obtain the differential expressed genes (DEGs). Next, using the STRING website, we performed the protein interaction network analysis. Subsequently, hub genes were screened using the R and Cytoscape software. Then, the expression difference and survival analyses of the hub genes were confirmed using GIPIA. Here, we established that the gene was correlated to the overall survival prognosis of CC. Besides, the expression of the gene in the GSE63514 dataset was significantly different from that of the normal cervix, cervical pre-cancerous lesions, and CC. Consequently, immunohistochemistry analysis showed that the results have a definite diagnostic value.
The gene could be linked with the pathophysiology of CC and maybe one of the early diagnostic markers for the diagnosis of cervical pre-cancerous lesions.
在全球范围内，宫颈癌（CC）是影响女性的第四大常见癌症。尽管有效的筛查降低了其发病率，但它仍然是威胁女性健康的最严重癌症之一。因此，本研究的目的是寻找可作为CC预后潜在生物标志物的新基因。
从基因表达综合数据库（GEO）下载GSE6791、GSE63678和GSE63514等三个数据集后，我们合并了表达矩阵并进行分析以获得差异表达基因（DEG）。接下来，使用STRING网站进行蛋白质相互作用网络分析。随后，使用R和Cytoscape软件筛选枢纽基因。然后，使用GIPIA确认枢纽基因的表达差异和生存分析。在此，我们确定该基因与CC的总生存预后相关。此外，该基因在GSE63514数据集中的表达与正常宫颈、宫颈癌前病变和CC的表达有显著差异。因此，免疫组织化学分析表明结果具有明确的诊断价值。
该基因可能与CC的病理生理学相关，可能是宫颈浸润癌前病变诊断的早期诊断标志物之一。
