抗菌纳米材料的纳入及其对根管封闭剂抗菌活性、流动性和射线不透过性的影响。
Incorporating Antimicrobial Nanomaterial and its Effect on the Antimicrobial Activity, Flow and Radiopacity of Endodontic Sealers.
作者信息
Teixeira Ana Beatriz Vilela, Vidal Carla Larissa, de Castro Denise Tornavoi, de Oliveira-Santos Christiano, Schiavon Marco Antônio, Dos Reis Andréa Cândido
机构信息
From the Department of Dental Materials and Prosthesis, University of São Paulo School of Dentistry of Ribeirão Preto, Ribeirão Preto, Brazil.
Department of Stomatology, Publich Health and Forensic Dentistry, University of São Paulo School of Dentistry of Ribeirão Preto, Ribeirão Preto, Brazil.
出版信息
Eur Endod J. 2017 Jul 6;2(1):1-6. doi: 10.14744/eej.2017.16029. eCollection 2017.
OBJECTIVE
This preliminary study aimed to evaluate the antimicrobial activity, flow and radiopacity of endodontic sealers with nanostructured silver vanadate decorated with silver nanoparticles (AgVO3).
METHODS
The minimum inhibitory concentration (MIC) of AgVO3 was evaluated against . Specimens were prepared from the following endodontic sealers: AH Plus (DENTSPLY DeTrey GmbH, Konstanz, Germany), Sealapex (Sybron Endo, Orange, CA, USA), Sealer 26 (DENTSPLY, Petrópolis, Brazil) and Endofill (DENTSPLY, Petrópolis, Brazil), with concentrations of 0%, 2.5%, 5% and 10% of AgVO3. Agar diffusion was used to evaluate the materials after 48 hours and 7 days (n=6). Flow (n=6) and radiopacity (n=9) were evaluated. The data were analysed by analysis of variance (ANOVA) and the Tukey honestly significant difference (HSD) (α=0.05).
RESULTS
The MIC of AgVO3 was 500 μg/mL for and 31.25 μg/mL for and . The AgVO3 did not influence the antimicrobial activity of AH Plus against (P>0.05) but did promote this activity for Sealapex (P<0.01). Moreover, this activity increased for Endofill from 2.5% and for Sealer 26 from 5% (P<0.05). Against , only AH Plus and Endofill 10% inhibited zone formation (P<0.01). The antimicrobial activity of Endofill increased from 2.5% against (P<0.01). Sealapex 5% and 10% (P<0.01), Sealer 26 10% and AH Plus promoted antimicrobial activity against E. coli. An increase in the zone of inhibition occurred between 48 hours and 7 days in the Sealapex 10% and Endofill 5% groups against E. coli. The flow of AH Plus and Endofill decreased with the increase of AgVO3 (P<0.05), and the flow of Sealer 26 and Sealapex was not affected (P>0.05). The radiopacity of AH Plus increased with AgVO3 (P<0.05). Endofill 5% and 10% did not differ from the control Endofill (P>0.05). The incorporation of AgVO3 did not influence the radiopacity of Sealer 26 (P>0.05). The incorporation of 2.5% and 5% AgVO3 reduced the radiopacity of Sealapex (P<0.05).
CONCLUSION
Adding AgVO3 may increase the antimicrobial effect of endodontic sealers without major changes in their physicochemical properties.
目的
本初步研究旨在评估用银纳米颗粒修饰的纳米结构钒酸银（AgVO₃）对根管封闭剂抗菌活性、流动性和射线不透性的影响。
方法
评估AgVO₃对……的最低抑菌浓度（MIC）。从以下根管封闭剂制备标本：AH Plus（德国康斯坦茨登士柏德特雷有限公司）、Sealapex（美国加利福尼亚州奥兰治赛峰根管公司）、Sealer 26（巴西彼得罗波利斯登士柏德公司）和Endofill（巴西彼得罗波利斯登士柏德公司），AgVO₃浓度分别为0%、2.5%、5%和10%。采用琼脂扩散法在48小时和7天后评估材料（n = 6）。评估流动性（n = 6）和射线不透性（n = 9）。数据采用方差分析（ANOVA）和Tukey真实显著差异（HSD）进行分析（α = 0.05）。
结果
AgVO₃对……的MIC为500μg/mL，对……和……为31.25μg/mL。AgVO₃不影响AH Plus对……的抗菌活性（P>0.05），但对Sealapex有促进作用（P<0.01）。此外，Endofill从2.5%开始、Sealer 26从5%开始抗菌活性增加（P<0.05）。针对……，只有AH Plus和10%的Endofill抑制抑菌圈形成（P<0.01）。Endofill对……的抗菌活性从2.5%开始增加（P<0.01）。5%和10%的Sealapex（P<0.01）、10%的Sealer 26和AH Plus对大肠杆菌有抗菌活性。在针对大肠杆菌的实验中，10%的Sealapex组和5%的Endofill组在48小时至7天之间抑菌圈增大。AH Plus和Endofill的流动性随AgVO₃增加而降低（P<0.05），Sealer 26和Sealapex的流动性不受影响（P>0.05）。AH Plus的射线不透性随AgVO₃增加（P<0.05）。5%和10%的Endofill与对照Endofill无差异（P>0.05）。加入AgVO₃不影响Sealer 26的射线不透性（P>0.05）。加入2.5%和5%的AgVO₃降低了Sealapex的射线不透性（P<0.05）。
结论
添加AgVO₃可能会增加根管封闭剂的抗菌效果，而其理化性质无重大变化。
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