Department of Dermatology, Venereology and Leprosy, PGIMER, Chandigarh, India.
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk, Rajasthan, 304022, India.
Arch Dermatol Res. 2021 Nov;313(9):759-767. doi: 10.1007/s00403-020-02168-w. Epub 2021 Jan 5.
In autoimmune onset of vitiligo, perilesional area shows inflammatory cells including T cytotoxic, helper cells and macrophages. Dendritic Cells (DCs) regulate immune activities by antigen presentation to T cells or cytokine production. It is evident that pro- and anti-inflammatory DCs are responsible for their respective cytokines release. However, role of DCs in vitiligo is enigmatic. In the present study, we assessed DCs markers (CD11b and CD11c) along with pro- and anti-inflammatory cytokines (IL-17A, IL-10 and IL-12p70) in stable and active vitiligo patients. Our results revealed a significant augmented expression of CD11bCD11c (pro-inflammatory DC) in peripheral blood mononuclear cells (PBMCs) and skin tissues of active vitiligo patients versus control and stable vitiligo group. Unlikely, CD11b (anti-inflammatory DC) levels were significantly impeded in active vitiligo group as compared to another two groups. CD11c (T helper 1 stimulating DC) presented no significant alterations in any group. Furthermore, we perceived significantly up-regulated IL-17A (pro-inflammatory cytokine) and down-regulated IL-10 (anti-inflammatory cytokine) expressions in active vitiligo group as compared to control and stable group (in sera, PBMCs and skin tissue). Also, a significant positive correlation was observed between CD11bCD11c and IL-17A; and CD11b and IL-10. Contrarily, CD11bCD11c and CD11b were negatively correlated with IL-10 and IL-17A, respectively. In conclusion, modulation of pro- and anti-inflammatory DCs in active vitiligo patients may affect cytokines production and thereby, lead to further depigmentation of skin.
在自身免疫性白癜风发病中，病变周围区域显示出包括 T 细胞毒性、辅助细胞和巨噬细胞在内的炎症细胞。树突状细胞（DCs）通过向 T 细胞呈递抗原或产生细胞因子来调节免疫活性。显然，促炎和抗炎 DCs 负责各自细胞因子的释放。然而，DCs 在白癜风中的作用仍然是一个谜。在本研究中，我们评估了稳定期和活动期白癜风患者的 DCs 标志物（CD11b 和 CD11c）以及促炎和抗炎细胞因子（IL-17A、IL-10 和 IL-12p70）。我们的结果表明，与对照组和稳定期白癜风组相比，活动期白癜风患者外周血单核细胞（PBMCs）和皮肤组织中 CD11bCD11c（促炎 DC）的表达显著增加。相反，与另外两组相比，活动期白癜风组中 CD11b（抗炎 DC）水平显著降低。CD11c（辅助 T 细胞刺激 DC）在任何一组中均无明显变化。此外，我们发现与对照组和稳定期组相比，活动期白癜风组中 IL-17A（促炎细胞因子）表达显著上调，IL-10（抗炎细胞因子）表达显著下调（血清、PBMCs 和皮肤组织）。此外，我们还观察到 CD11bCD11c 与 IL-17A 之间呈显著正相关；CD11b 与 IL-10 之间也呈显著正相关。相反，CD11bCD11c 与 CD11b 分别与 IL-10 和 IL-17A 呈负相关。总之，活动期白癜风患者促炎和抗炎 DCs 的调节可能会影响细胞因子的产生，从而导致皮肤进一步脱色。
