Department of Infectious Diseases and Clinical Microbiology, Sanliurfa Training and Research Hospital, Sanliurfa, Turkey.
Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Harran University, Sanliurfa, Turkey.
Int J Clin Pract. 2021 May;75(5):e13988. doi: 10.1111/ijcp.13988. Epub 2021 Feb 3.
To assess the dynamic thiol/disulphide homeostasis (DTDH) and total oxidant/antioxidant status in patients with hepatitis C virus (HCV) infection and to evaluate their association with HCV-RNA levels.
Levels of serum total thiol (TT), native thiol (NT), disulphide (DS), total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) as oxidative stress markers were determined in 162 individuals, including 74 patients with HCV infection and 88 non-HCV controls. HCV genotypes and HCV-RNA levels of the patients were recorded.
The NT, TT and TAS levels and NT/TT ratio were significantly lower in the HCV group compared with the control group. On the contrary, DS, TOS and OSI levels and DS/NT and DS/TT ratios were significantly higher. Patients with high HCV RNA levels (> 650 000 IU/mL) had higher DS levels than patients with low HCV-RNA levels (<650 000 IU/mL). Genotype 1 was observed in 68.9% of patients with HCV. Levels of oxidative stress parameters were similar between genotype 1 and other genotypes (2, 3 and 5). No significant correlations were found between oxidative stress markers and albumin, alanine aminotransferase, aspartate aminotransferase, bilirubin and HCV-RNA levels in patients with HCV infection. A negative correlation was found only between OSI and albumin.
Our results suggest that DTDH shifts towards the DS direction because of thiol oxidation in HCV-infected patients. Furthermore, DS levels were significantly higher in patients with high HCV-RNA levels compared with patients with low HCV-RNA levels.
评估丙型肝炎病毒（HCV）感染患者的动态硫醇/二硫键动态平衡（DTDH）和总氧化剂/抗氧化剂状态，并评估它们与 HCV-RNA 水平的关系。
测定了 162 例个体的血清总巯基（TT）、天然巯基（NT）、二硫化物（DS）、总氧化剂状态（TOS）、总抗氧化状态（TAS）和氧化应激指数（OSI）等氧化应激标志物水平，包括 74 例 HCV 感染患者和 88 例非 HCV 对照者。记录了患者的 HCV 基因型和 HCV-RNA 水平。
与对照组相比，HCV 组的 NT、TT 和 TAS 水平以及 NT/TT 比值明显降低。相反，DS、TOS 和 OSI 水平以及 DS/NT 和 DS/TT 比值明显升高。HCV-RNA 水平较高（>650 000 IU/mL）的患者 DS 水平高于 HCV-RNA 水平较低（<650 000 IU/mL）的患者。HCV 患者中观察到基因型 1 占 68.9%。基因型 1 与其他基因型（2、3 和 5）之间的氧化应激参数水平相似。在 HCV 感染患者中，未发现氧化应激标志物与白蛋白、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、胆红素和 HCV-RNA 水平之间存在显著相关性。仅发现 OSI 与白蛋白呈负相关。
我们的结果表明，由于 HCV 感染患者的巯基氧化，DTDH 向 DS 方向转移。此外，与 HCV-RNA 水平较低的患者相比，HCV-RNA 水平较高的患者 DS 水平显著升高。
