Liang Xinjin, Zhang Shuai, Gadd Geoffrey Michael, McGrath John, Rooney David W, Zhao Qi
The Bryden Centre, School of Chemical and Chemistry Engineering, Queen's University Belfast, Univeristy Road, Belfast BT7 1NN, UK.
Geomicrobiology Group, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.
Regen Biomater. 2022 Feb 22;9(1):rbac013. doi: 10.1093/rb/rbac013. eCollection 2022.
Pin-tract infections (PTIs) are a common complication of external fixation of fractures and current strategies for preventing PTIs have proven to be ineffective. Recent advances show that the use of anti-infection coatings with local antibacterial activity may solve this problem. Selenium has been considered as a promising anti-infection agent owing to its antibacterial and antibiofilm activities. In this study, selenium nanoparticles (SeNPs) were synthesized a cost-effective fungi-mediated biorecovery approach and demonstrated excellent stability and homogeneity. To investigate their anti-infection potential, the SeNPs were doped in silver coatings through an electroless plating process and the silver-selenium (Ag-Se) coatings were tested for antibacterial and antibiofilm properties against F1557 and WT F1693 as well as corrosion resistance in simulated body fluid. It was found that the Ag-Se coating significantly inhibited growth and biofilm formation on the surface, reducing 81.2% and 59.7% of viable bacterial adhesion when compared with Ag and Ag-PTFE-coated surfaces after 3 days. The Ag-Se coating also exhibited improved corrosion resistance compared with the Ag coating, leading to a controlled release of Ag, which in turn reduced the risk of cytotoxicity against hFOBs. These results suggest that the fungal-derived SeNPs may have potential in use as implant coatings to prevent PTIs.
针道感染（PTIs）是骨折外固定的常见并发症，目前预防针道感染的策略已被证明无效。最新进展表明，使用具有局部抗菌活性的抗感染涂层可能解决这一问题。由于其抗菌和抗生物膜活性，硒已被视为一种有前景的抗感染剂。在本研究中，通过一种经济高效的真菌介导生物回收方法合成了硒纳米颗粒（SeNPs），并证明其具有出色的稳定性和均匀性。为了研究其抗感染潜力，通过化学镀将SeNPs掺杂到银涂层中，并测试了银 - 硒（Ag - Se）涂层对F1557和野生型F1693的抗菌和抗生物膜性能以及在模拟体液中的耐腐蚀性。结果发现，与银和银 - 聚四氟乙烯涂层表面相比，Ag - Se涂层在3天后显著抑制了表面细菌生长和生物膜形成，使活菌粘附减少了81.2%和59.7%。与银涂层相比，Ag - Se涂层的耐腐蚀性也有所提高，导致银的可控释放，进而降低了对人成骨细胞（hFOBs）的细胞毒性风险。这些结果表明，真菌衍生的SeNPs作为植入物涂层预防针道感染可能具有潜力。
