Magnetic Silicium Hydroxyapatite Nanorods for Enhancing Osteoblast Response in Vitro and Biointegration in Vivo.

Osteoblast behavior playing an important role in the biointegration of the Ti implant with host bone in vivo can be regulated by surface properties and magnetic field. In order to endow the Ti surface with good osteogenesis activity, Si monosubstituted and Fe and Si cosubstituted hydroxyapatite (HAp) nanorods were fabricated on microporous TiO by microarc oxidation (MAO) followed with hydrothermal treatment (HT). The surface properties including microstructure, microroughness, hydrophilicity, ion release, magnetic property, cytocompatibility, and biointegration of substituted HAp nanorods were observed and evaluated, together with pure HAp nanorods and microarc oxidated (MAOed) TiO as controls. After being doped with Fe, MAOed TiO has no changes in phase composition and microroughness, whereas it displays weakly ferromagnetic behavior and can enhance osteoblast differentiation in vitro and formation of new bone in vivo, compared with the undoped one. The substituted HAp nanorods adhere firmly to TiO and have almost the same wettability and microroughness but additional Si, Fe, and/or Ca released into the medium, compared with pure HAp nanorods. Moreover, the cosubstituted HAp has a small ferromagnetic signal, while its saturation magnetization value is less than that of the MAOed doped with Fe. Compared to pure HA nanorods, the substituted HAp nanorods not only improve cell proliferation and differentiation in vitro, but also enhance the ability of bone integration in vivo, especially for the cosubstituted one, which should be ascribed to the combined effect of microstructure, magnetic property, and released ions.