Lu Denghua, Yang Xudong, Zhang Qingrong, Wang Ruixia, Zhou Siyuan, Yang Guocheng, Shan Yuping
School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China.
ACS Biomater Sci Eng. 2019 Feb 11;5(2):432-437. doi: 10.1021/acsbiomaterials.8b01363. Epub 2018 Dec 24.
Carbon dots (CDs) evoke a great deal of attention in biomedicine because of their unique properties. As an emerging theranostic agent, the CDs transmembrane transport process is the first and the most important step. Herein, a transmembrane dynamic process of transporting single CDs with folic acid into normal cells (Vero) and cancer cells (HeLa) were tracked by means of a force tracing technique based on atomic force microscopy. Meanwhile, the kinetic parameters of the the transmembrane process were measured and calculated. Interestingly, in the comparison between cancer cells (HeLa) and normal cells (Vero), the transporting force of single CDs with folic acid was smaller and the transporting average speed was slower.
碳点（CDs）因其独特的性质而在生物医学领域引起了广泛关注。作为一种新兴的诊疗剂，碳点的跨膜运输过程是首要且最重要的一步。在此，基于原子力显微镜的力追踪技术对携带叶酸的单个碳点跨膜进入正常细胞（Vero）和癌细胞（HeLa）的动态过程进行了追踪。同时，测量并计算了跨膜过程的动力学参数。有趣的是，在癌细胞（HeLa）与正常细胞（Vero）的比较中，携带叶酸的单个碳点的运输力更小，运输平均速度更慢。
