Chen Xi, Tian Falin, Li Min, Xu Haijiao, Cai Mingjun, Li Qian, Zuo Xiaolei, Wang Hongda, Shi Xinghua, Fan Chunhai, Baigude Huricha, Shan Yuping
School of Chemistry and Life Science Advanced Institute of Materials Science Changchun University of Technology Changchun Jilin 130012 China.
School of Chemistry & Chemical Engineering Inner Mongolia Key Laboratory of Mongolian Medicinal Chemistry Inner Mongolia University Hohhot Inner Mongolia 010020 China.
Glob Chall. 2019 Nov 20;4(3):1900075. doi: 10.1002/gch2.201900075. eCollection 2020 Mar.
DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force-tracing technique based on atomic force microscopy. The results show that transporting single TDNs into living HeLa cells need ≈53 pN force and ≈25 ms duration with the average speed of ≈0.6 µm s. Interestingly, the dynamic parameters are irrelevant to the size of TDNs, while the larger TDNs rotated slightly during the transporting process. Meanwhile, both the results from single-molecule force tracing and ensemble fluorescence imaging demonstrate that the different size TDNs transmembrane transporting depends on caveolin-mediated endocytosis.
DNA纳米结构作为药物递送载体已引起了广泛关注。然而，DNA纳米结构的跨膜动力学仍有待深入研究。在此，基于原子力显微镜的力追踪技术实时监测单个四面体DNA纳米结构（TDN）的转运动态过程。结果表明，将单个TDN转运到活的HeLa细胞中需要约53 pN的力和约25 ms的持续时间，平均速度约为0.6 µm/s。有趣的是，这些动态参数与TDN的大小无关，而较大的TDN在转运过程中会轻微旋转。同时，单分子力追踪和整体荧光成像的结果均表明，不同大小的TDN跨膜转运依赖于小窝蛋白介导的内吞作用。
