Yang Yu, Zhang Qingrong, Cai Mingjun, Xu Haijiao, Lu Denghua, Liu Yulin, Fu Yanfeng, Yang Guocheng, Shan Yuping
School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Yan'an St. 2055, Changchun 130012, China.
Changchun Institute of Applied Chemistry, Chinese Academy of Science, Renmin St. 5625, Changchun 130022, China.
ACS Omega. 2020 Apr 21;5(17):9864-9869. doi: 10.1021/acsomega.0c00079. eCollection 2020 May 5.
Gold nanocages (Au NCs), as drug carriers, have been widely applied for cancer diagnosis and photothermal therapy (PTT). Transmembrane transporting efficacy of Au NCs is the fundamental and important issue for their use in PTT. Herein, we used a force tracing technique based on atomic force microscopy to track the dynamic transmembrane process of Au NCs at the single-particle level in real time. Meanwhile, we measured and compared the dynamic parameters of Au NCs with sizes of 50 and 100 nm usually used as nanodrug carriers of PTT. It is concluded that the 50 nm Au NC transmembrane transporting needs smaller force and shorter duration with a much faster speed. However, both the 50 and 100 nm Au NC transmembrane transporting depends on the caveolin-mediated endocytosis, clathrin-mediated endocytosis, and macropinocytosis, which was also confirmed by confocal fluorescence imaging. This report will provide a potential technique for screening nanodrug carriers from the perspective of transmembrane transporting efficacy.
金纳米笼（Au NCs）作为药物载体，已被广泛应用于癌症诊断和光热疗法（PTT）。Au NCs的跨膜转运效率是其用于PTT的基本且重要的问题。在此，我们使用基于原子力显微镜的力追踪技术，实时在单颗粒水平追踪Au NCs的动态跨膜过程。同时，我们测量并比较了通常用作PTT纳米药物载体的尺寸为50和100 nm的Au NCs的动态参数。结果表明，50 nm的Au NC跨膜转运所需的力更小、持续时间更短且速度更快得多。然而，50和100 nm的Au NC跨膜转运均依赖于小窝蛋白介导的内吞作用、网格蛋白介导的内吞作用和巨胞饮作用，共聚焦荧光成像也证实了这一点。本报告将从跨膜转运效率的角度为筛选纳米药物载体提供一种潜在技术。
