State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China.
College of Marine Science, Shandong University, Weihai 264209, PR China.
Int J Syst Evol Microbiol. 2020 Oct;70(10):5373-5381. doi: 10.1099/ijsem.0.004421. Epub 2020 Sep 4.
A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated CWB-1, was isolated from a haloalkaline lake sediment sample collected from the bottom of Chaiwopu Lake, Urumchi, Xinjiang Province, PR China. Strain CWB-1 grew at 4-40 °C (optimum, 30-35 °C), pH 6.5-9.0 (optimum, pH 6.5-7.0) and with 0.5-5.5 % (w/v) NaCl (optimum, 2.5-3.0 %). Phylogenetic analyses based on the 16S rRNA gene sequence and the whole genome sequence both revealed that strain CWB-1 belonged to the family . The strain had the highest similarity of the 16S rRNA gene sequence to PAMC 27130 (92.8 %). The genome of strain CWB-1 was 3 548 011 bp long with 36.3 % DNA G+C content. The predominant fatty acids (>10 %) in the CWB-1 cells were iso-C, iso-C 3-OH and summed feature 1 (iso-C H/C 3-OH). The major respiratory quinone was menaquinone-6 and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. Based on the phylogenetic analyses, as well as the phenotypic characteristics, a novel genus and species of the family , gen. nov., sp. nov., is proposed. The type strain is CWB-1 (=KCTC 72450=CGMCC 1.17149).
一株革兰氏阴性、严格需氧、不运动、杆状的细菌，被命名为 CWB-1，从中国新疆乌鲁木齐柴窝堡湖湖底的一个盐湖沉积物样本中分离得到。CWB-1 菌株在 4-40°C（最佳温度为 30-35°C）、pH 值 6.5-9.0（最佳 pH 值为 6.5-7.0）和 0.5-5.5%（w/v）NaCl（最佳 2.5-3.0%）下生长。基于 16S rRNA 基因序列和全基因组序列的系统发育分析均表明，CWB-1 菌株属于 科。该菌株与 PAMC 27130（92.8%）的 16S rRNA 基因序列具有最高的相似性。CWB-1 菌株的基因组全长为 3548011bp，GC 含量为 36.3%。CWB-1 细胞中主要的脂肪酸（>10%）为 iso-C、iso-C 3-OH 和总和特征 1（iso-C H/C 3-OH）。主要的呼吸醌是menaquinone-6，主要的极性脂类是磷脂酰乙醇胺、一种未鉴定的胺脂类和两种未鉴定的脂类。基于系统发育分析以及表型特征，提出了一个新的科、属、种，即. 属，nov. ，种，nov. 。该模式菌株为 CWB-1（=KCTC 72450=CGMCC 1.17149）。
