Ali Sardar, Xie Jianmin, Chen Yuerong, Cai Runlin, Juventus Aweya Jude, Hu Zhong, Zhang Yueling, Wang Hui
Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou 511458, PR China.
Biology Department and Institute of Marine Sciences, College of Science, and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, PR China.
Int J Syst Evol Microbiol. 2021 Oct;71(10). doi: 10.1099/ijsem.0.005047.
A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterium (designated as LMIT005) was isolated from shrimp ponds in Shantou, China. The new isolate was characterized taxonomically using a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain LMIT005 was found to be affiliated with the family of the order while appearing as a distinct lineage. The 16S rRNA gene sequence similarity between strain LMIT005 and KCTC 12309, the closest type strain in the family, was 91.3 %. Strain LMIT005 grew optimally at 25 °C, pH 7 and in the presence of 2.0 % (w/v) NaCl. The DNA G+C content (data from genome sequence) was 40.5 mol%. Compared with reference strain KCTC 12309, the average nucleotide identity (ANI) of LMIT005 was 70 %. The sole respiratory quinone of LMIT005 was menaquinone (MK-7), and the major fatty acids were summed feature 3 (C ω6c / C ω7c). The polar lipids of strain LMIT005 were mainly composed of phosphatidylethanolamine, phosphatidylcholine, two unidentified amino lipids, two unidentified lipids, one unidentified glycolipid and one unidentified phospholipid. The draft genome of strain LMIT005 comprised 3 089 781 bp (3.09 Mb) nucleotides and 2773 genes. Antimicrobial resistant-related genes ( and ) were annotated in the genome of strain LMIT005, which indicated that it might be able to resist β-lactam antibiotics. This was further verified by antimicrobial resistant test. Given its distinct genomic, morphological, and physiological differences from previously described type strains, strain LMIT005 is proposed as a representative of a novel genus of the family with the name gen. nov., sp. nov. The type strain is LMIT005 (=KCTC 82616=CICC 25047).
从中国汕头的虾塘中分离出一株革兰氏染色阴性、无运动性、杆状、需氧细菌（命名为LMIT005）。采用多相分类法对该新分离菌株进行分类学特征分析。基于16S rRNA基因序列分析，发现菌株LMIT005隶属于该目下的科，但呈现为一个独特的谱系。菌株LMIT005与该科中最接近的模式菌株KCTC 12309的16S rRNA基因序列相似性为91.3%。菌株LMIT005在25 °C、pH 7以及2.0%（w/v）NaCl存在的条件下生长最佳。DNA G+C含量（来自基因组序列数据）为40.5 mol%。与参考菌株KCTC 12309相比，LMIT005的平均核苷酸同一性（ANI）为70%。LMIT005唯一的呼吸醌为甲基萘醌（MK-7），主要脂肪酸为总和特征3（C ω6c / C ω7c）。菌株LMIT005的极性脂质主要由磷脂酰乙醇胺、磷脂酰胆碱、两种未鉴定的氨基脂质、两种未鉴定的脂质、一种未鉴定的糖脂和一种未鉴定的磷脂组成。菌株LMIT005的基因组草图由3 089 781 bp（3.09 Mb）核苷酸和2773个基因组成。在菌株LMIT005的基因组中注释到了与抗菌抗性相关的基因（和），这表明它可能能够抵抗β-内酰胺类抗生素。抗菌抗性试验进一步证实了这一点。鉴于其与先前描述的模式菌株在基因组、形态和生理方面存在明显差异，菌株LMIT005被提议作为该科一个新属的代表，命名为新属，新种。模式菌株为LMIT005（=KCTC 82616=CICC 25047）。
