Department of Biophysics, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey.
Department of Biophysics, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey.
Biol Trace Elem Res. 2021 Nov;199(11):4154-4161. doi: 10.1007/s12011-020-02538-5. Epub 2021 Jan 6.
Trace element metabolism plays an important role in the formation of diabetes and complications of diabetes. Although trace elements changes in lenses in diabetic cataract and glaucoma formation have been investigated, there were few studies evaluating trace elements levels in plasma and aqueous humor fluid in diabetic and non-diabetic conditions. Therefore, we aimed to investigate zinc (Zn), copper (Cu), and chromium (Cr) levels in plasma and aqueous humor fluids of rabbits in the diabetic rabbit model. New Zealand male rabbits were divided into two groups as control (n = 8), and diabetes (n = 8) induced by alloxane. At the end of the experimental period, the osmolality in blood, plasma, and aqueous humor fluids from rabbits were measured by osmometer and Zn, Cu, and Cr levels in plasma and aqueous humor fluid were measured by inductively coupled plasma-optical emission spectrophotometer (ICP-OES). The osmolality in blood, plasma, and aqueous humor fluid of the diabetic group was significantly increased compared to the control group (respectively p < 0.01, p < 0.001, p < 0.001). It was analyzed that plasma Zn and Cu levels of diabetic rabbits increased significantly (respectively, p < 0.01; p < 0.001), whereas Cr level significantly decreased according to the control group (p < 0.01). It was observed that Cr and Zn levels in aqueous humor fluid in diabetes group decreased (respectively p < 0.001 and p < 0.01), and a significantly increased in Cu level (p < 0.001) compared to the control group. Related with these changes that may occur in the eye due to the measured parameters, we consider that comparative studies of these types of diabetic animal models would be useful in the evaluation of diabetes and its complications.
微量元素代谢在糖尿病的形成及其并发症中起着重要作用。虽然已经研究了糖尿病性白内障和青光眼形成过程中晶状体中微量元素的变化，但很少有研究评估糖尿病和非糖尿病状态下血浆和房水中微量元素的水平。因此，我们旨在研究糖尿病兔模型中兔血浆和房水中锌（Zn）、铜（Cu）和铬（Cr）的水平。新西兰雄性兔分为两组：对照组（n=8）和用阿脲诱导的糖尿病组（n=8）。在实验期末，通过渗透压计测量兔血、血浆和房水的渗透压，通过电感耦合等离子体-光学发射光谱仪（ICP-OES）测量血浆和房水中 Zn、Cu 和 Cr 的水平。与对照组相比，糖尿病组的血、血浆和房水渗透压显著增加（分别为 p<0.01、p<0.001 和 p<0.001）。分析发现糖尿病兔的血浆 Zn 和 Cu 水平显著增加（分别为 p<0.01 和 p<0.001），而 Cr 水平则显著降低（p<0.01）。观察到糖尿病组房水中的 Cr 和 Zn 水平降低（分别为 p<0.001 和 p<0.01），Cu 水平显著升高（p<0.001），与对照组相比。考虑到这些变化可能与所测量的参数有关，我们认为对这些类型的糖尿病动物模型进行比较研究有助于评估糖尿病及其并发症。
