Jia Ru, Sadiq Faizan Ahmed, Liu Wenbin, Cao Lirong, Shen Zhuoyu
School of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, Shanxi, China.
School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Food Chem Toxicol. 2021 Feb;148:111962. doi: 10.1016/j.fct.2020.111962. Epub 2021 Jan 4.
Deoxynivalenol (DON) poses a serious health threat to animals and humans consuming DON-contaminated food and feed. Biological means of detoxification of DON are considered as one of the effective strategies. The aim of the work was to study ameliorative effects of Bacillus subtilis ASAG 216 on DON-induced toxicosis in piglets. A decrease in average daily gain and average daily feed intake was observed in piglets fed DON-contaminated feed. In addition, DON exposure increased the serum concentrations of aspartate aminotransferase, immunoglobulin A, diamine oxidase, endotoxin, and peptide YY. Moreover, DON exposure caused oxidative stress in the serum, liver and jejunum, induced intestinal inflammation, impaired the intestinal barrier, and disturbed the gut microbiota homeostasis. Supplementation of B. subtilis ASAG 216 effectively attenuated the aforementioned effects of DON on piglets. Moreover, DON and de-epoxy-DON (DOM-1) in the serum, liver and kidney were significantly decreased when B. subtilis ASAG 216 was added to DON-contaminated diet. Our results imply that B. subtilis ASAG 216 can protect against DON-induced toxicosis in piglets, and thus this strain has a potential to be used as an animal feed ingredient to counteract harmful effects of DON in animals.
脱氧雪腐镰刀菌烯醇（DON）对食用受DON污染的食物和饲料的动物及人类构成严重的健康威胁。DON的生物解毒方法被认为是有效的策略之一。这项工作的目的是研究枯草芽孢杆菌ASAG 216对DON诱导的仔猪中毒的改善作用。在喂食受DON污染饲料的仔猪中观察到平均日增重和平均日采食量下降。此外，接触DON会增加血清中天冬氨酸转氨酶、免疫球蛋白A、二胺氧化酶、内毒素和肽YY的浓度。此外，接触DON会导致血清、肝脏和空肠中的氧化应激，引发肠道炎症，损害肠道屏障，并扰乱肠道微生物群稳态。补充枯草芽孢杆菌ASAG 216可有效减轻DON对仔猪的上述影响。此外，当在受DON污染的日粮中添加枯草芽孢杆菌ASAG 216时，血清、肝脏和肾脏中的DON和脱环氧-DON（DOM-1）显著降低。我们的结果表明，枯草芽孢杆菌ASAG 216可以预防DON诱导的仔猪中毒，因此该菌株有潜力用作动物饲料成分，以抵消DON对动物的有害影响。
