Habib M P, Eskelson C, Katz M A
Tucson Veterans Administration Medical Center, AZ 85723.
Am Rev Respir Dis. 1988 Feb;137(2):341-4. doi: 10.1164/ajrccm/137.2.341.
Production rates for ethane gas, a hydrocarbon byproduct of lipid peroxidation, measured from the ethane present in the exhaled breath of rats, were used to determine changes in oxygen-free radical activity. Rates of ethane production were measured in rats housed in metabolic chambers and exposed to room air and to high oxygen concentrations. Expired ethane, adsorbed onto activated charcoal and then liberated by heating, was measured by gas chromatography. Ethane production rates in groups of 8 rats increased during 8 h of 100% oxygen exposure from a mean (+/- SE) room air value of 11.30 +/- 1.15 to 27.85 +/- 2.93 pmol/min/100 g rat (p less than 0.005). The mean (+/- SE) percent increases in ethane production after exposure to 100% oxygen in 8 rats pretreated with 0.1 mg/100 g of vitamin E in corn oil vehicle (0.1 ml/100 g) injected intraperitoneally and in a group of 5 rats injected with vehicle alone averaged 157.16 +/- 37.83% and 150.98 +/- 25.19%, respectively. The percent changes noted were not significantly different as measured by analysis of variance. These data indicate that lipid peroxidative activity, hence oxygen-free radical activity, increases in normally fed rats exposed to hyperoxia at a time prior to the previously reported neutrophil influx into the lungs of similarly exposed rats and that it is not attenuated by pretreatment with vitamin E.
从大鼠呼出气体中的乙烷含量测得的乙烷气体生成率（乙烷是脂质过氧化的一种碳氢化合物副产物），被用于确定氧自由基活性的变化。在代谢笼中饲养并暴露于室内空气和高氧浓度环境下的大鼠，测量其乙烷生成率。呼出的乙烷被吸附到活性炭上，然后通过加热释放出来，用气相色谱法进行测量。在100%氧气暴露8小时期间，8只大鼠一组的乙烷生成率从室内空气环境下的平均（±标准误）值11.30±1.15增加到27.85±2.93皮摩尔/分钟/100克大鼠（p<0.005）。预先经腹腔注射0.1毫克/100克玉米油载体（0.1毫升/100克）维生素E处理的8只大鼠和仅注射载体的5只大鼠一组，在暴露于100%氧气后，乙烷生成率平均增加的百分比分别为157.16±37.83%和150.98±25.19%。通过方差分析测量，观察到的百分比变化没有显著差异。这些数据表明，在正常喂养的大鼠中，暴露于高氧环境时，脂质过氧化活性（即氧自由基活性）在先前报道的中性粒细胞流入类似暴露大鼠肺部之前就已增加，并且用维生素E预处理并不能使其减弱。
