Diaz-Lara Alfredo, Dangl Gerald, Yang Jydy, Golino Deborah Anne, Al Rwahnih Maher
University of California, Dept. of Plant Pathology, One Shields Avenue, Davis, California, United States, 95616;
University of California Davis, Foundation Plant Services, Davis, California, United States;
Plant Dis. 2021 Jan 8. doi: 10.1094/PDIS-10-20-2121-SC.
Grapevine Pinot gris virus (GPGV) is a recently identified pathogen of grapevines in California. To advance our knowledge about the epidemiology of GPGV, we investigated if free-living Vitis spp. can represent a source of virus infection. In 2019 a field survey of GPGV infection was conducted in Napa County. During the inspection 60 free-living vines in riparian habitats near commercial vineyards with GPGV infection were sampled. Samples were tested by real-time reverse transcription PCR (RT-PCR), identifying 23 free-living Vitis spp. positive for GPGV. Later, GPGV infection was confirmed in these plants via end-point RT-PCR and Sanger sequencing. Based on sequence analysis, detected GPGV isolates are more related to the asymptomatic variant of the virus. Vitis species ancestry was determined by DNA fingerprinting. GPGV-infected material included V. californica, V. californica × V. vinifera hybrids and hybrid rootstock cultivars. Here, GPGV is reported for the first time in free-living Vitis spp. The results of this study will support the development of management strategies for GPGV in California and beyond.
葡萄灰比诺病毒（GPGV）是加利福尼亚州最近发现的一种葡萄病原体。为了增进我们对GPGV流行病学的了解，我们调查了野生葡萄属植物是否可能是病毒感染源。2019年，在纳帕县对GPGV感染情况进行了实地调查。在检查过程中，从靠近感染GPGV的商业葡萄园的河岸栖息地采集了60株野生葡萄藤样本。通过实时逆转录PCR（RT-PCR）对样本进行检测，鉴定出23株野生葡萄属植物GPGV呈阳性。随后，通过终点RT-PCR和桑格测序在这些植物中确认了GPGV感染。基于序列分析，检测到的GPGV分离株与该病毒的无症状变体关系更为密切。通过DNA指纹图谱确定了葡萄属物种的谱系。感染GPGV的材料包括加州葡萄、加州葡萄×酿酒葡萄杂交种和杂交砧木品种。在此，首次报道了野生葡萄属植物感染GPGV。本研究结果将有助于制定加利福尼亚州及其他地区GPGV的管理策略。
