在商业葡萄园附近河岸地区的野生葡萄属植物中鉴定出灰比诺葡萄病毒。

Identification of grapevine Pinot gris virus in free-living Vitis spp. located in riparian areas adjacent to commercial vineyards.

作者信息

Diaz-Lara Alfredo, Dangl Gerald, Yang Jydy, Golino Deborah Anne, Al Rwahnih Maher

机构信息

University of California, Dept. of Plant Pathology, One Shields Avenue, Davis, California, United States, 95616;

University of California Davis, Foundation Plant Services, Davis, California, United States;

出版信息

Plant Dis. 2021 Jan 8. doi: 10.1094/PDIS-10-20-2121-SC.

DOI:10.1094/PDIS-10-20-2121-SC

PMID:33417499

Abstract

Grapevine Pinot gris virus (GPGV) is a recently identified pathogen of grapevines in California. To advance our knowledge about the epidemiology of GPGV, we investigated if free-living Vitis spp. can represent a source of virus infection. In 2019 a field survey of GPGV infection was conducted in Napa County. During the inspection 60 free-living vines in riparian habitats near commercial vineyards with GPGV infection were sampled. Samples were tested by real-time reverse transcription PCR (RT-PCR), identifying 23 free-living Vitis spp. positive for GPGV. Later, GPGV infection was confirmed in these plants via end-point RT-PCR and Sanger sequencing. Based on sequence analysis, detected GPGV isolates are more related to the asymptomatic variant of the virus. Vitis species ancestry was determined by DNA fingerprinting. GPGV-infected material included V. californica, V. californica × V. vinifera hybrids and hybrid rootstock cultivars. Here, GPGV is reported for the first time in free-living Vitis spp. The results of this study will support the development of management strategies for GPGV in California and beyond.

摘要

葡萄灰比诺病毒（GPGV）是加利福尼亚州最近发现的一种葡萄病原体。为了增进我们对GPGV流行病学的了解，我们调查了野生葡萄属植物是否可能是病毒感染源。2019年，在纳帕县对GPGV感染情况进行了实地调查。在检查过程中，从靠近感染GPGV的商业葡萄园的河岸栖息地采集了60株野生葡萄藤样本。通过实时逆转录PCR（RT-PCR）对样本进行检测，鉴定出23株野生葡萄属植物GPGV呈阳性。随后，通过终点RT-PCR和桑格测序在这些植物中确认了GPGV感染。基于序列分析，检测到的GPGV分离株与该病毒的无症状变体关系更为密切。通过DNA指纹图谱确定了葡萄属物种的谱系。感染GPGV的材料包括加州葡萄、加州葡萄×酿酒葡萄杂交种和杂交砧木品种。在此，首次报道了野生葡萄属植物感染GPGV。本研究结果将有助于制定加利福尼亚州及其他地区GPGV的管理策略。