Vacca Carla, Contu Maria Paola, Rossi Cecilia, Ferrando Maria Laura, Blus Cornelio, Szmukler-Moncler Serge, Scano Alessandra, Orrù Germano
Molecular Biology Service (MBS), Department of Surgical Sciences, University of Cagliari, 09124 Cagliari, Italy.
Private Dental Practice, 22100 Como, Italy.
Pathogens. 2020 Dec 20;9(12):1069. doi: 10.3390/pathogens9121069.
Peri-implantitis is a steadily rising disease and is caused by oral bacterial pathogens able to form biofilm on implant surfaces and peri-implant tissues, making antibiotics treatment less effective. The use of commercial probiotics against oral pathogens could serve as an alternative to prevent biofilm formation. is one of the early colonizers of biofilm formation in dental implants. The aim of this study was to model the interaction between and strain K12, a probiotic bacterium producing bacteriocins. was co-cultured with K12 in an in vitro model simulating the biofilm formation in a dental implant composed by a titanium cylinder system. Biofilm formation rate was assessed by Real-Time PCR quantification of bacterial count and expression levels of gene, used in response to cell density in the biofilm. Biofilm formation, bacteriocin production, expression patterns were found to be already expressed within the first 12 h. More importantly, K12 was able to counter the biofilm formation in a titanium cylinder under the tested condition. In conclusion, our dental implant model may be useful for exploring probiotic-pathogen interaction to find an alternative to antibiotics for peri-implantitis treatment.
种植体周围炎是一种发病率持续上升的疾病，由能够在种植体表面和种植体周围组织形成生物膜的口腔细菌病原体引起，这使得抗生素治疗效果不佳。使用商业益生菌对抗口腔病原体可作为预防生物膜形成的一种替代方法。[具体细菌名称1]是牙种植体生物膜形成的早期定植菌之一。本研究的目的是模拟[具体细菌名称1]与[具体细菌名称2]K12（一种产生细菌素的益生菌）之间的相互作用。在一个体外模型中，将[具体细菌名称1]与[具体细菌名称2]K12共同培养，该模型模拟了由钛圆柱体系统组成的牙种植体中的生物膜形成。通过实时定量PCR对细菌计数和用于响应生物膜中细胞密度的[具体基因名称]基因表达水平进行评估，以评估生物膜形成率。发现生物膜形成、细菌素产生、[具体基因名称]表达模式在最初12小时内就已出现。更重要的是，在测试条件下，[具体细菌名称2]K12能够对抗钛圆柱体中的生物膜形成。总之，我们的牙种植体模型可能有助于探索益生菌与病原体之间的相互作用，以寻找替代抗生素治疗种植体周围炎的方法。
