一种用于在活细胞中鉴别检测半胱氨酸、同型半胱氨酸和谷胱甘肽的内质网靶向荧光探针。
An endoplasmic reticulum-targeting fluorescent probe for discriminatory detection of Cys, Hcy and GSH in living cells.
作者信息
Yue Xiuxiu, Chen Jiali, Chen Wenqiang, Wang Benhua, Zhang Hui, Song Xiangzhi
机构信息
College of Chemistry & Chemical Engineering, Central South University, Changsha, Hunan 410083, China.
College of Chemistry and Materials Science, Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning, Guangxi 530001, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Apr 5;250:119347. doi: 10.1016/j.saa.2020.119347. Epub 2020 Dec 22.
Endoplasmic reticulum, known to us as the ER, is the largest organelle in many kinds of eukaryotic cells and plays vital roles in maintaining the normal function of cells. Biothiols (Cys, Hcy, GSH) in secretory proteins will be modified as they enter the ER and are of great importance in balancing redox state of ER environments. In this article, we have developed the first endoplasmic reticulum-targeting fluorescent probe, ER-CP, for concurrent distinguishment of Cys, Hcy and GSH with favorable sensitivity and selectivity. ER-CP was successfully used in fluorescence imaging of Cys, Hcy and GSH in HeLa cells. In addition, ER-CP exhibited a good ER-targeting property (Pearson's correlation coefficient = 0.90).
内质网，我们都知道它简称为ER，是多种真核细胞中最大的细胞器，在维持细胞正常功能方面发挥着至关重要的作用。分泌蛋白中的生物硫醇（半胱氨酸、同型半胱氨酸、谷胱甘肽）在进入内质网时会被修饰，这对于平衡内质网环境的氧化还原状态非常重要。在本文中，我们开发了首个靶向内质网的荧光探针ER-CP，用于同时区分半胱氨酸、同型半胱氨酸和谷胱甘肽，具有良好的灵敏度和选择性。ER-CP成功用于HeLa细胞中半胱氨酸、同型半胱氨酸和谷胱甘肽的荧光成像。此外，ER-CP表现出良好的内质网靶向特性（皮尔逊相关系数=0.90）。
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