Microelectronics Research & Develop Center, Shanghai University, Shanghai, China.
Key Laboratory of Advanced Display and System Applications, Ministry of Education, Shanghai University, Shanghai, China.
PLoS One. 2020 Apr 29;15(4):e0231981. doi: 10.1371/journal.pone.0231981. eCollection 2020.
An acetylcholinesterase biosensor modified with graphene and transition metal carbides was prepared to detect organophosphorus pesticides. Cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy were used to characterize the electrochemical catalysis of the biosensor: acetylcholinesterase/chitosan-transition metal carbides/graphene/glassy carbon electrode. With the joint modification of graphene and transition metal carbides, the biosensor has a good performance in detecting dichlorvos with a linear relationship from 11.31 μM to 22.6 nM and the limit of detection was 14.45 nM. Under the premise of parameter optimization, the biosensor showed a good catalytic performance for acetylcholine. Compared to the biosensors without modification, it expressed a better catalytic performance due to the excellent electrical properties, biocompatibility and high specific surface area of graphene, transition metal carbides. Finally, the biosensor exhibits good stability, which can be stored at room temperature for one month without significant performance degradation, and has practical potential for sample testing.
一种基于石墨烯和过渡金属碳化物修饰的乙酰胆碱酯酶生物传感器被制备用于检测有机磷农药。循环伏安法、差分脉冲伏安法和电化学阻抗谱被用于对生物传感器的电化学催化作用进行表征：乙酰胆碱酯酶/壳聚糖-过渡金属碳化物/石墨烯/玻碳电极。通过石墨烯和过渡金属碳化物的联合修饰，该生物传感器在检测敌敌畏时具有良好的性能，线性关系范围为 11.31 μM 至 22.6 nM，检测限为 14.45 nM。在参数优化的前提下，该生物传感器对乙酰胆碱表现出良好的催化性能。与未经修饰的生物传感器相比，由于石墨烯、过渡金属碳化物具有优异的导电性、生物相容性和高比表面积，该生物传感器表现出更好的催化性能。最后，该生物传感器表现出良好的稳定性，在室温下可储存一个月而性能无明显下降，具有实际的样品检测潜力。
