Sakudo Akikazu, Yagyu Yoshihito
School of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, Japan.
Laboratory of Biometabolic Chemistry, School of Health Sciences, University of the Ryukyus, Nishihara, Okinawa, Japan.
AMB Express. 2021 Jan 9;11(1):16. doi: 10.1186/s13568-020-01177-2.
Efficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressure dielectric barrier discharge (APDBP) plasma on a set of rollers. Penicillium venetum was spotted onto the surface of the fruit or pericarps, as well as an aluminium plate to act as a control, before performing the plasma treatment. The results showed that viable cell number of P. venetum decreased with a decimal reduction time (D value or estimated treatment time required to reduce viable cell number by 90%) of 0.967 min on the aluminium plate, 2.90 min and 1.88 min on the pericarps of 'Kiyomi' (Citrus unshiu × C. sinensis) and 'Kawano-natsudaidai' (C. natsudaidai) respectively, and 2.42 min on the surface of 'Unshu-mikan' (C. unshiu). These findings confirmed a fungicidal effect of the plasma not only on an abiotic surface (aluminium plate) but also on a biotic surface (citrus fruit). Further development of the instrument by combining sorting systems with the plasma device promises an efficient means of disinfecting citrus fruits during food processing.
需要高效的方法来实现农产品的安全去污。在此，我们研究了柑橘类水果的去污情况，以测试一种新型非热气体等离子体装置——滚筒输送式等离子体仪器的抗真菌潜力。该仪器在一组滚筒上产生大气压介质阻挡放电（APDBP）等离子体。在进行等离子体处理之前，将威尼斯青霉点涂在水果或果皮表面以及铝板上作为对照。结果表明，威尼斯青霉的活菌数在铝板上以0.967分钟的十进制减少时间（D值，即活菌数减少90%所需的估计处理时间）下降，在“清见”（温州蜜柑×甜橙）和“川野夏橙”（夏橙）的果皮上分别为2.90分钟和1.88分钟，在“温州蜜柑”表面为2.42分钟。这些发现证实了等离子体不仅对非生物表面（铝板）有杀菌作用，对生物表面（柑橘类水果）也有杀菌作用。通过将分选系统与等离子体装置相结合对该仪器进行进一步开发，有望成为食品加工过程中对柑橘类水果进行消毒的有效手段。
