Koppolu Pradeep, Sirisha Sunkara, Mishra Ashank, Deshpande Kopparesh, Lingam Amara Swapna, Alotaibi Dalal H, Saleh Alwahibi Mohammed, Penela Soumya
Department of Preventive Dental Sciences, College of Dentistry, Dar Al Uloom University, Riyadh, Saudi Arabia.
Consultant Periodontist, Clove Dental Hospital, Hyderabad, India.
Saudi J Biol Sci. 2021 Jan;28(1):380-385. doi: 10.1016/j.sjbs.2020.10.016. Epub 2020 Oct 14.
Periodontitis is commonly diagnosed based on clinical parameters. However, the analysis of a few unique biomarkers of the disease process present in the saliva and blood can further assist the estimation of the rate of disease progression.
The present study attempted to correlate the alkaline phosphatase (ALP) and acid phosphatase (ACP) levels in saliva and serum between patients with healthy periodontium, gingivitis, and chronic periodontitis.
The present study was conducted in 135 subjects between 20 and 55 years of age. The subjects were divided into three groups, namely healthy (Group A), gingivitis (Group B), and chronic periodontitis (Group C). The clinical parameters were recorded using the plaque index (PI), gingival index (GI), and probing depth (PD). Saliva and serum were analyzed for ALP and ACP levels using an auto analyzer. All patients underwent scaling and root planning (SRP) along with oral hygiene instructions. Patients were then recalled after four weeks, and blood and saliva samples were collected to estimate ALP and ACP levels prior to clinical examination.
The clinical parameters exhibited a statistically significant decrease in the PI and GI in both group B and group C after SRP. A significant change in the PD and attachment levels (AL) was observed in the periodontitis group after SRP. The mean salivary & serum ALP levels exhibited a statistically significant decrease in group B & C after SRP. The mean serum ACP levels exhibited a statistically significant decrease in group B & C after SRP However, the salivary ACP levels decrease after SRP was only statistically significant in group C.
Serum and salivary ALP and ACP levels were markedly decreased in the gingivitis and periodontitis groups after SRP and were positively correlated with the clinical parameters.
牙周炎通常根据临床参数进行诊断。然而，分析唾液和血液中存在的该疾病过程的一些独特生物标志物可以进一步辅助估计疾病进展速度。
本研究试图关联健康牙周组织、牙龈炎和慢性牙周炎患者唾液和血清中的碱性磷酸酶（ALP）和酸性磷酸酶（ACP）水平。
本研究在135名年龄在20至55岁之间的受试者中进行。受试者分为三组，即健康组（A组）、牙龈炎组（B组）和慢性牙周炎组（C组）。使用菌斑指数（PI）、牙龈指数（GI）和探诊深度（PD）记录临床参数。使用自动分析仪分析唾液和血清中的ALP和ACP水平。所有患者均接受了龈上洁治和根面平整（SRP）以及口腔卫生指导。然后在四周后召回患者，在临床检查前采集血液和唾液样本以估计ALP和ACP水平。
SRP后，B组和C组的临床参数PI和GI均有统计学意义的下降。SRP后，牙周炎组的PD和附着水平（AL）有显著变化。SRP后，B组和C组的唾液和血清ALP平均水平有统计学意义的下降。SRP后，B组和C组的血清ACP平均水平有统计学意义的下降。然而，SRP后唾液ACP水平的下降仅在C组有统计学意义。
SRP后，牙龈炎和牙周炎组的血清和唾液ALP及ACP水平显著降低，且与临床参数呈正相关。
