Al-Yousef Hanan M, Alqahtani Ali S, Ghani Afaf S A, El-Toumy Sayed A, El-Dougdoug Wagdi I A, Hassan Wafaa H B, Hassan Hanaa M
Pharmacognosy Department, College of Pharmacy, King Saud University, POBox 2457, Riyadh 11451, Saudi Arabia.
Department of Pharmacognosy Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Egypt.
Saudi J Biol Sci. 2021 Jan;28(1):785-792. doi: 10.1016/j.sjbs.2020.11.011. Epub 2020 Nov 11.
To investigate the effect of the ethyl acetate fraction of the aerial parts of L F. on nephroprotective, cytotoxic, and antioxidant.
different spectroscopic and spectrophotometric methods were applied to identify phytoconstituents. The nephroprotective potential of ethyl acetate fraction ( EtOAc) was evaluated in male rats with thioacetamide-induced kidney injury, as wll as cytotoxic activity was evaluated using a viability assay, and the antioxidant activity was evaluated using the DPPH method. Results: quantitative estimation of total phenolics and flavonoids of was performed using unique spectrophotometric methods. The polyphenolic compounds gallic acid , ellagic acid , kaempferol-3--(6″--galloyl--glucopyranoside) , quercetin-3---glucopyranoside and quercetin-3----arabinoside were isolated from the ethyl acetate fraction of the aerial parts of . The thioacetamide administration resulted in marked nephrotoxicity, but pretreatment with EtOAc significantly attenuated the nephrotoxicity through alteration of kidney biomarkers, thereby improving the redox status of the tissue and restoring serum biochemical parameters nearly to normal levels. This study revealed a significant cytotoxic and strong antioxidant effect. Conclusion: we conclude that the EtOAc may be used in the future as nephroprotective, cytotoxic, and antioxidant agent derived from a natural source.
研究某植物地上部分乙酸乙酯部位对肾脏的保护作用、细胞毒性及抗氧化作用。
采用不同的光谱和分光光度法鉴定植物成分。在硫代乙酰胺诱导的肾损伤雄性大鼠中评估乙酸乙酯部位（EtOAc）的肾脏保护潜力，通过活力测定评估细胞毒性活性，并采用DPPH法评估抗氧化活性。结果：采用独特的分光光度法对某植物的总酚和黄酮进行定量测定。从某植物地上部分的乙酸乙酯部位分离出多酚化合物没食子酸、鞣花酸、山奈酚 - 3 - O -（6″ - O - 没食子酰基 - β - 吡喃葡萄糖苷）、槲皮素 - 3 - O - β - 葡萄糖苷和槲皮素 - 3 - O - α - 阿拉伯糖苷。硫代乙酰胺给药导致明显的肾毒性，但用EtOAc预处理可通过改变肾脏生物标志物显著减轻肾毒性，从而改善组织的氧化还原状态并使血清生化参数恢复至接近正常水平。本研究揭示了显著的细胞毒性和强大的抗氧化作用。结论：我们得出结论，EtOAc未来可能作为一种源自天然来源的肾脏保护、细胞毒性和抗氧化剂使用。
