Jadrane Issam, Al Feddy Mohamed Najib, Dounas Hanane, Kouisni Lamfeddal, Aziz Faissal, Ouahmane Lahcen
Laboratory of Microbial Biotechnologies, Agrosciences and Environment, Faculty of Science Semlalia, Cadi Ayyad University, Marrakesh, Morocco.
Phytobacteriology Laboratory, Plant Protection Research Unit, CRRA Marrakesh, National Institute for Agronomical Research, Marrakesh, Morocco.
Saudi J Biol Sci. 2021 Jan;28(1):825-832. doi: 10.1016/j.sjbs.2020.11.018. Epub 2020 Nov 11.
In the current study, we investigated the impact of inoculation with a selected indigenous arbuscular mycorrhizal fungi (AMF) complex on the growth and physiology of carob plants at increasing levels of watering (25, 50, 75 and 100% field capacity). The following growth and stress parameters were monitored in carob seedlings after 6 months of growth and 2 months of applied drought stress: fresh and dry weight, root and shoot lengths, leaf surface area, relative water content, stomatal conductance and membrane stability. Chlorophyll a and b, total soluble sugars, proline and protein contents were also determined along with the activities of stress enzymes: Catalase, Peroxidase and Superoxide dismutase. The obtained results indicate that inoculation with the indigenous AMF complex has a positive impact on the plant's growth as all the assessed parameters were significantly improved in the mycorrhizal plants. Additionally, our results show that mycorrhization contributes to the minimization of the impact of drought stress on the carob plants and allows a better adaptation to dry conditions.
在当前研究中，我们调查了接种特定的本土丛枝菌根真菌（AMF）复合体对在不同浇水水平（田间持水量的25%、50%、75%和100%）下角豆树植株生长和生理的影响。在角豆树幼苗生长6个月并施加2个月干旱胁迫后，监测了以下生长和胁迫参数：鲜重和干重、根长和茎长、叶面积、相对含水量、气孔导度和膜稳定性。还测定了叶绿素a和b、总可溶性糖、脯氨酸和蛋白质含量以及胁迫酶（过氧化氢酶、过氧化物酶和超氧化物歧化酶）的活性。所得结果表明，接种本土AMF复合体对植株生长有积极影响，因为在菌根化植株中所有评估参数均显著改善。此外，我们的结果表明，菌根化有助于将干旱胁迫对角豆树植株的影响降至最低，并使其更好地适应干旱条件。
