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全局调控因子Talae1的过表达导致从内生真菌中发现新的抗真菌聚酮化合物。

Overexpression of Global Regulator Talae1 Leads to the Discovery of New Antifungal Polyketides From Endophytic Fungus .

作者信息

Ding Zhuang, Wang Xiao, Kong Fan-Dong, Huang Hui-Ming, Zhao Yan-Na, Liu Min, Wang Zheng-Ping, Han Jun

机构信息

Institute of BioPharmaceutical Research, Liaocheng University, Liaocheng, China.

Hainan Key Laboratory for Research and Development of Natural Product From Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.

出版信息

Front Microbiol. 2020 Dec 23;11:622785. doi: 10.3389/fmicb.2020.622785. eCollection 2020.

DOI:10.3389/fmicb.2020.622785
PMID:33424824
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7785522/
Abstract

Transcription regulation caused by global regulators exerts important effects on fungal secondary metabolism. By overexpression of the global regulator Talae1 in a -associated fungus , two structurally new polyketides ( and ) that were newly produced in the transformant were isolated and identified. Their structures, including the absolute configurations, were elucidated through a combination of high resolution mass spectrometer (HRMS), NMR, and electronic circular dichroism (ECD) calculations. The growth inhibitory activities of compounds and were evaluated against four bacteria and six plant-pathogenic fungi. Compound showed the highest antifungal activity against and f. sp. with MIC of 8 μg/ml. To the best of our knowledge, this is the first study to report on the application of the global regulator in to activate the biosynthesis of bioactive secondary metabolites.

摘要

由全局调控因子引起的转录调控对真菌次级代谢产生重要影响。通过在一种相关真菌中过表达全局调控因子Talae1，分离并鉴定了转化体中新产生的两种结构新颖的聚酮化合物（和）。通过高分辨率质谱仪（HRMS）、核磁共振（NMR）和电子圆二色性（ECD）计算相结合的方法阐明了它们的结构，包括绝对构型。评估了化合物和对四种细菌和六种植物病原真菌的生长抑制活性。化合物对和f. sp.表现出最高的抗真菌活性，最低抑菌浓度（MIC）为8μg/ml。据我们所知，这是第一项报道在中应用全局调控因子激活生物活性次级代谢产物生物合成的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/dd846f281c71/fmicb-11-622785-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/18031e7022ee/fmicb-11-622785-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/087ba20fd655/fmicb-11-622785-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/eabea8c88ee2/fmicb-11-622785-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/661e6052bde1/fmicb-11-622785-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/3b2ae9a5edaa/fmicb-11-622785-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/dd846f281c71/fmicb-11-622785-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/18031e7022ee/fmicb-11-622785-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/087ba20fd655/fmicb-11-622785-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/eabea8c88ee2/fmicb-11-622785-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/661e6052bde1/fmicb-11-622785-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/3b2ae9a5edaa/fmicb-11-622785-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2256/7785522/dd846f281c71/fmicb-11-622785-g006.jpg

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2
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Front Chem. 2020 Apr 21;8:270. doi: 10.3389/fchem.2020.00270. eCollection 2020.
3
Epigenetic Agents Trigger the Production of Bioactive Nucleoside Derivatives and Bisabolane Sesquiterpenes From the Marine-Derived Fungus .
内生真菌：抗真菌代谢物的宝库。
Microorganisms. 2024 Sep 18;12(9):1903. doi: 10.3390/microorganisms12091903.
4
Involvement of LaeA and Velvet Proteins in Regulating the Production of Mycotoxins and Other Fungal Secondary Metabolites.LaeA和Velvet蛋白在调控霉菌毒素及其他真菌次生代谢产物合成中的作用
J Fungi (Basel). 2024 Aug 8;10(8):561. doi: 10.3390/jof10080561.
5
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6
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J Xenobiot. 2023 Oct 1;13(4):572-603. doi: 10.3390/jox13040037.
7
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8
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