Thomas Nurain, Rusdin Agus, Tulsyahra Madania, Wathoni Nasrul, Kuswandi Asep
Department of Pharmacy, Faculty of Sports and Health, Universitas Negeri Gorontalo, Gorontalo, Indonesia.
Department of Pharmaceutical and Technology Pharmaceutics, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Indonesia.
J Adv Pharm Technol Res. 2020 Oct-Dec;11(4):226-232. doi: 10.4103/japtr.JAPTR_11_20. Epub 2020 Oct 10.
(JTS), an important fluid carried in xylem and phloem tubes of L. plant, has good wound healing property. However, physicochemical stability of JTS needs to be improved in order for it to be useful as a topical wound-healing agent. In this study, we developed an iota carrageenan-polyvinyl alcohol (IC-PVA) hydrogel film (HF) as a carrier of JTS and evaluated its wound-healing ability. The characterization of JTS secondary metabolites by ultraviolet-Vis spectrophotometry suggested presence of flavonoid, saponin, and alkaloids in the sap. We successfully extracted IC from using alkaline solvent at 80°C-90°C with calcium chloride as the precipitator. The result of computer simulation using Discovery Studio software and Autodock Tools showed the presence of hydrogen bonding interaction of IC-PVA. IC-PVA/JTS HF with excellent physical properties including high swelling ratio (246.32%) and high gel fraction (16.75%). In addition, irritation test in mice confirmed the absence of hypersensitivity reaction, redness, and allergic reactions. Interestingly, IC-PVA/JTS HF significantly accelerated wound healing when compared to the nontreated group/control with 98% wound closure by 10 days. These results suggest that IC-PVA HF has improves wound-healing ability of JTS.
植物乳杆菌木质部和韧皮部导管中携带的一种重要液体（JTS）具有良好的伤口愈合特性。然而，为了使其作为局部伤口愈合剂有用，JTS的物理化学稳定性需要提高。在本研究中，我们开发了一种ι-卡拉胶-聚乙烯醇（IC-PVA）水凝胶膜（HF）作为JTS的载体，并评估了其伤口愈合能力。通过紫外-可见分光光度法对JTS次生代谢产物的表征表明，汁液中存在黄酮类、皂苷类和生物碱类。我们在80°C-90°C下使用碱性溶剂并以氯化钙作为沉淀剂成功地从……中提取了IC。使用Discovery Studio软件和Autodock Tools进行的计算机模拟结果显示了IC-PVA的氢键相互作用。IC-PVA/JTS HF具有优异的物理性能，包括高溶胀率（246.32%）和高凝胶分数（16.75%）。此外，小鼠刺激性试验证实没有过敏反应、发红和过敏反应。有趣的是，与未处理组/对照组相比，IC-PVA/JTS HF显著加速了伤口愈合，到第10天时伤口闭合率达到98%。这些结果表明，IC-PVA HF提高了JTS的伤口愈合能力。
