Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, 21934, Egypt.
Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), Alexandria, Egypt.
Sci Rep. 2021 Oct 14;11(1):20456. doi: 10.1038/s41598-021-00069-0.
Wound healing is a complicated multicellular process that involves several kinds of cells including macrophages, fibroblasts, endothelial cells, keratinocytes and platelets that are leading to their differentiation towards an anti-inflammatory response for producing several chemokines, cytokine and growth factors. In this study, electrospun nanofiber scaffold named (MNS) is composed of polyvinyl alcohol (PVA)/iota carrageenan (IC) and doped with partially reduced graphene oxide (prGO) that is successfully synthesized for wound healing and skin repair. The fabricated MNS was tested in case of infection and un-infection with E. coli and Staphylococcus and in both of the presence and in the absence of yeast as a natural nutritional supplement. Numerous biochemical parameters including total protein, albumin, urea and LDH, and hematological parameters were evaluated. Results revealed that the MNS was proved to be effective on most of the measured parameters and had exhibited efficient antibacterial inhibition activity. Whereas it can be used as an effective antimicrobial agent in wound healing, however, histopathological findings confirmed that the MNS caused re-epithelialization and the presence of yeast induced hair follicles growth and subsequently it may be used to hide formed head wound scar.
伤口愈合是一个复杂的多细胞过程，涉及多种细胞，包括巨噬细胞、成纤维细胞、内皮细胞、角质细胞和血小板，它们会分化为抗炎反应，产生多种趋化因子、细胞因子和生长因子。在这项研究中，成功合成了一种名为（MNS）的静电纺纳米纤维支架，由聚乙烯醇（PVA）/角叉菜胶（IC）组成，并掺杂部分还原氧化石墨烯（prGO），可用于伤口愈合和皮肤修复。测试了制备的 MNS 在感染和未感染大肠杆菌和金黄色葡萄球菌的情况下，以及在存在和不存在酵母作为天然营养补充剂的情况下的效果。评估了包括总蛋白、白蛋白、尿素和 LDH 在内的许多生化参数以及血液学参数。结果表明，MNS 对大多数测量参数都有效，并表现出有效的抗菌抑制活性。尽管它可以作为伤口愈合的有效抗菌剂，但组织病理学发现证实，MNS 可促进上皮再形成，而酵母的存在可诱导毛囊生长，随后可用于掩盖形成的头部伤口疤痕。
