Bakaic Emilia, Smeets Niels M B, Badv Maryam, Dodd Megan, Barrigar Owen, Siebers Emily, Lawlor Michael, Sheardown Heather, Hoare Todd
Department of Pathology and Laboratory Medicine and Neuroscience Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, United States.
ACS Biomater Sci Eng. 2018 Nov 12;4(11):3713-3725. doi: 10.1021/acsbiomaterials.7b00397. Epub 2017 Sep 12.
Injectable, dual-responsive, and degradable poly(oligo ethylene glycol methacrylate) (POEGMA) hydrogels are demonstrated to offer potential for cell delivery. Charged groups were incorporated into hydrazide and aldehyde-functionalized thermoresponsive POEGMA gel precursor polymers via the copolymerization of N,'-dimethylaminoethyl methacrylate (DMAEMA) or acrylic acid (AA) to create dual-temperature/pH-responsive in situ gelling hydrogels that can be injected via narrow gauge needles. The incorporation of charge significantly broadens the swelling, degradation, and rheological profiles achievable with injectable POEGMA hydrogels without significantly increasing nonspecific protein adsorption or chronic inflammatory responses following in vivo subcutaneous injection. However, significantly different cell responses are observed upon charge incorporation, with charged gels significantly improving 3T3 mouse fibroblast cell adhesion in 2D and successfully delivering viable and proliferating ARPE-19 human retinal epithelial cells via an "all-synthetic" matrix that does not require the incorporation of cell-adhesive peptides.
可注射、双响应且可降解的聚（甲基丙烯酸寡聚乙二醇酯）（POEGMA）水凝胶被证明在细胞递送方面具有潜力。通过甲基丙烯酸N，N'-二甲氨基乙酯（DMAEMA）或丙烯酸（AA）的共聚，将带电基团引入到酰肼和醛功能化的热响应性POEGMA凝胶前体聚合物中，以制备双温度/pH响应的原位凝胶化水凝胶，该水凝胶可通过细针注射。电荷的引入显著拓宽了可注射POEGMA水凝胶的溶胀、降解和流变特性，且在体内皮下注射后不会显著增加非特异性蛋白质吸附或慢性炎症反应。然而，引入电荷后观察到显著不同的细胞反应，带电凝胶显著改善了二维条件下3T3小鼠成纤维细胞的黏附，并通过一种“全合成”基质成功递送了有活力且能增殖的ARPE-19人视网膜上皮细胞，该基质无需掺入细胞黏附肽。
