Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4L8, Canada.
ACS Biomater Sci Eng. 2021 Sep 13;7(9):4258-4268. doi: 10.1021/acsbiomaterials.0c01552. Epub 2021 Feb 11.
Reactive electrospinning is demonstrated as a viable method to create fast-responsive and degradable macroporous thermoresponsive hydrogels based on poly(oligoethylene glycol methacrylate) (POEGMA). Hydrazide- and aldehyde-functionalized POEGMA precursor polymers were coelectrospun to create hydrazone cross-linked nanostructured hydrogels in a single processing step that avoids the need for porogens, phase separation-driving additives, or scaffold postprocessing. The resulting nanostructured hydrogels can respond reversibly and repeatedly to changes in external temperature within seconds, in contrast to the minutes-to-hours response time observed with bulk hydrogels. Furthermore, nearly quantitative cell delamination can be achieved within 2 min of incubation at 4 °C, resulting in the recovery of as many or more (as well as more proliferatively active) cells from the substrate relative to the conventional trypsinization protocol. The combined macroporosity, nanoscale feature size, and interfacial switching potential of these nanostructured hydrogels thus offer promise for manipulating cell-hydrogel interactions as well as other applications in which rapid responses to external stimuli are desirable.
反应性静电纺丝被证明是一种可行的方法，可以基于聚（聚乙二醇甲基丙烯酸酯）（POEGMA）来制备快速响应和可降解的大孔热响应水凝胶。将酰肼和醛官能化的 POEGMA 前体聚合物共静电纺丝，以在单个处理步骤中形成腙交联的纳米结构水凝胶，从而避免了使用致孔剂、相分离驱动添加剂或支架后处理的需要。所得的纳米结构水凝胶可以在几秒钟内对外界温度的变化进行可逆和重复的响应，而与体积水凝胶观察到的分钟到小时的响应时间相比。此外，在 4°C 孵育 2 分钟内可以实现几乎定量的细胞分层，与传统的胰蛋白酶处理方案相比，可以从基质中回收更多或更多（以及更具增殖活性）的细胞。这些纳米结构水凝胶的大孔率、纳米级特征尺寸和界面切换潜力相结合，因此有望用于操纵细胞-水凝胶相互作用以及其他需要对外界刺激快速响应的应用。
