Smeets Niels M B, Bakaic Emilia, Patenaude Mathew, Hoare Todd
Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L8, Canada.
Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L8, Canada.
Acta Biomater. 2014 Oct;10(10):4143-55. doi: 10.1016/j.actbio.2014.05.035. Epub 2014 Jun 7.
The potential of poly(oligoethylene glycol methacrylate) (POEGMA) hydrogels with varying thermosensitivities as soft materials for biomedical applications is demonstrated. Hydrogels are prepared from hydrazide and aldehyde functionalized POEGMA precursors, yielding POEGMA hydrogels with a volume phase transition temperature (VPTT) below (PO0), close to (PO10) and well above (PO100) physiological temperature. Hydrogels with VPTTs close to and above physiological temperature exhibit biological properties similar to those typically observed for poly(ethylene glycol) hydrogels (i.e. low protein adsorption, low cell adhesion and minimal inflammatory responses in vivo) while hydrogels with VPTTs lower than physiological temperature exhibit biological properties more analogous to poly(N-isopropylacrylamide) above its phase transition temperature (temperature-switchable cell adhesion, higher protein adsorption and somewhat more acute inflammation in vivo). As such, the use of POEGMA precursors with varying chain lengths of ethylene oxide grafts offers a versatile platform for the design of hydrogels with tunable physiological properties via simple copolymerization.
本文展示了具有不同热敏感性的聚（甲基丙烯酸寡聚乙二醇酯）（POEGMA）水凝胶作为生物医学应用软材料的潜力。水凝胶由酰肼和醛功能化的POEGMA前体制备而成，得到体积相变温度（VPTT）低于生理温度（PO0）、接近生理温度（PO10）以及远高于生理温度（PO100）的POEGMA水凝胶。VPTT接近和高于生理温度的水凝胶表现出与聚（乙二醇）水凝胶通常观察到的生物特性相似（即低蛋白吸附、低细胞粘附和体内最小炎症反应），而VPTT低于生理温度的水凝胶表现出与高于其相变温度的聚（N-异丙基丙烯酰胺）更相似的生物特性（温度可切换的细胞粘附、更高的蛋白吸附和体内稍更剧烈的炎症）。因此，使用具有不同环氧乙烷接枝链长度的POEGMA前体，通过简单的共聚作用，为设计具有可调生理特性的水凝胶提供了一个通用平台。
