Datta Sayanti, Rameshbabu Arun Prabhu, Bankoti Kamakshi, Maity Priti Prasanna, Das Dipankar, Pal Sagar, Roy Sabyasachi, Sen Ramkrishna, Dhara Santanu
Department of Applied Chemistry, Indian School of Mines, Dhanbad 826004, India.
Department of Gynaecology, Midnapore Medical College, Paschim Medinipur 721101, India.
ACS Biomater Sci Eng. 2017 Aug 14;3(8):1738-1749. doi: 10.1021/acsbiomaterials.7b00189. Epub 2017 Jul 19.
Wound healing management is a major challenge for critical full-thickness skin wounds. Development of nanofibrous scaffolds with tunable wettability, degradation, and biocompatibility are highly desirable. Herein, we demonstrated synthesis of oleoyl chitosan (OC) by grafting monounsaturated fatty acid residue, C oleoyl chain, to the backbone of chitosan molecule and blending with gelatin to form the nanofiber mats. The physicochemical properties of the nanofiber mats revealed mechanical strength, moderate surface wettability, and suitable degradation rate. The nanofibrous mats showed excellent in vitro cytocompatibility with human amniotic membrane-derived stem cells (HAMSCs) in terms of enhanced adhesion and proliferation owing to biomimetic nanoarchitecture and chemical cues. Furthermore, the fabricated nanofiber was implanted with and without preseeded HAMSCs in the full-thickness wound to evaluate the skin wound healing efficacy in a rat model. Histological and immunohistochemical studies were conducted to evaluate the plausible changes of tissue architecture and expression of molecular markers involved in wound healing process. Both acellular and HAMSCs incorporated cellular nanofibers promoted wound contraction remarkably with superior skin tissue regeneration in terms of enhanced collagen synthesis, re-epithelialization and initiation of epithelial cells stratification compared to control group.
伤口愈合管理是严重全层皮肤伤口面临的一项重大挑战。开发具有可调节润湿性、降解性和生物相容性的纳米纤维支架非常必要。在此，我们展示了通过将单不饱和脂肪酸残基（油酰基链）接枝到壳聚糖分子主链上并与明胶共混以形成纳米纤维垫来合成油酰基壳聚糖（OC）。纳米纤维垫的物理化学性质显示出机械强度、适度的表面润湿性和合适的降解速率。由于仿生纳米结构和化学信号，纳米纤维垫在增强人羊膜来源干细胞（HAMSCs）的粘附和增殖方面表现出与HAMSCs优异的体外细胞相容性。此外，将制备的纳米纤维在全层伤口中植入有无预先接种HAMSCs的情况，以评估大鼠模型中的皮肤伤口愈合效果。进行了组织学和免疫组织化学研究，以评估伤口愈合过程中组织结构的可能变化和涉及的分子标志物的表达。与对照组相比，无细胞和含有HAMSCs的细胞纳米纤维均显著促进伤口收缩，并在增强胶原蛋白合成、再上皮化和上皮细胞分层起始方面具有优异的皮肤组织再生能力。
