State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Key Laboratory of Agro-Biodiversity and Pest Management of Ministry of Education, Yunnan Agricultural University, Kunming, 650201, China.
BMC Plant Biol. 2021 Jan 11;21(1):40. doi: 10.1186/s12870-020-02821-6.
Some of the pathogenic effector proteins play an active role in stimulating the plant defense system to strengthen plant resistance.
In this study, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was implemented to identify altered metabolites in transgenic rice containing over-expressed M. oryzae Systemic Defense Trigger 1 (MoSDT1) that was infected at three-time points. The characterized dominating metabolites were organic acids and their derivatives, organic oxygen compounds, lipids, and lipid-like molecules. Among the identified metabolites, shikimate, galactinol, trehalose, D-mannose, linolenic acid, dopamine, tyramine, and L-glutamine are precursors for the synthesis of many secondary defense metabolites Carbohydrate, as well as amino acid metabolic, pathways were revealed to be involved in plant defense responses and resistance strengthening.
The increasing salicylic acid (SA) and jasmonic acid (JA) content enhanced interactions between JA synthesis/signaling gene, SA synthesis/receptor gene, raffinose/fructose/sucrose synthase gene, and cell wall-related genes all contribute to defense response in rice. The symptoms of rice after M. oryzae infection were significantly alleviated when treated with six identified metabolites, i.e., galactol, tyramine, L-glutamine, L-tryptophan, α-terpinene, and dopamine for 72 h exogenously. Therefore, these metabolites could be utilized as an optimal metabolic marker for M. oryzae defense.
一些病原效应蛋白在激活植物防御系统以增强植物抗性方面发挥着积极作用。
本研究采用超高效液相色谱-四极杆飞行时间质谱（UHPLC/Q-TOF-MS）鉴定了转 M. oryzae 系统防御触发蛋白 1（MoSDT1）基因过量表达的水稻在感染三个时间点后发生变化的代谢物。鉴定出的主要代谢物为有机酸及其衍生物、有机含氧化合物、脂质和类脂分子。在鉴定出的代谢物中，莽草酸、半乳糖醇、海藻糖、D-甘露糖、亚麻酸、多巴胺、酪胺和 L-谷氨酰胺是许多次生防御代谢物合成的前体，碳水化合物以及氨基酸代谢途径参与了植物防御反应和抗性增强。
水杨酸（SA）和茉莉酸（JA）含量的增加增强了 JA 合成/信号基因、SA 合成/受体基因、棉子糖/果糖/蔗糖合酶基因和细胞壁相关基因之间的相互作用，所有这些都有助于水稻的防御反应。用 6 种鉴定出的代谢物（半乳糖醇、酪胺、L-谷氨酰胺、L-色氨酸、α-萜品烯和多巴胺）对水稻进行 72 h 外源处理后，稻瘟病菌感染后的症状明显缓解。因此，这些代谢物可以作为稻瘟病防御的最佳代谢标志物。
