Davari Nader, Ahmadpour Fatemeh, Kiani Ali Asghar, Azadpour Mozhgan, Asadi Zari Tahannejad
Thalassemia & Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
BMC Res Notes. 2021 Jan 11;14(1):21. doi: 10.1186/s13104-020-05428-0.
In chronic lymphocytic leukemia (CLL), lack of expression or dysregulation of some special miRs disrupts apoptosis of malignant cells; thereby miR expression can enhance cell proliferation, disease progression and decrease patient survival.
30 CLL patients and 20 healthy individuals participated in the study. RNA was extracted to evaluate the expression of miR-125, miR-223, BCL-2 and signal transducer and transcription 3 activator (STAT3) genes; quantitative Real Time- PCR (Q-RT-PCR) was performed. MiR-125a and miR-223 expression decreased in the patients compared to the control group (P-Value:0.001). BCL-2 and STAT3 which are the target genes of these two miRs, showed increased expression, in the patients compared to the control subjects (P-Value: 0.001 and P-Value: 0.64 respectively). A significant reverse relationship was found between miR-125a and BCl-2 expression and WBC count. Significantly, miR-223 expression was associated with smoking in patients (P-Value: 0.007). Also, these miRs may have regulatory effects by controlling white blood cell (WBC) production based on the inverse correlation with WBC count and hemoglobin (Hb) concentration. Finally, miR-223 can be used as a prognostic factor in CLL patients; miR-125a may be useful for evaluating the therapeutic approaches based on the inverse link with BCl-2.
在慢性淋巴细胞白血病（CLL）中，某些特定微小RNA（miR）的表达缺失或失调会破坏恶性细胞的凋亡；因此，miR表达可增强细胞增殖、疾病进展并降低患者生存率。
30例CLL患者和20名健康个体参与了该研究。提取RNA以评估miR - 125、miR - 223、BCL - 2和信号转导及转录激活因子3（STAT3）基因的表达；进行了定量实时聚合酶链反应（Q - RT - PCR）。与对照组相比，患者中miR - 125a和miR - 223表达降低（P值：0.001）。这两种miR的靶基因BCL - 2和STAT3在患者中的表达相较于对照受试者增加（P值分别为0.001和0.64）。在miR - 125a与BCl - 2表达和白细胞计数之间发现了显著的负相关关系。值得注意的是，患者中miR - 223表达与吸烟相关（P值：0.007）。此外，基于与白细胞计数和血红蛋白（Hb）浓度的负相关，这些miR可能通过控制白细胞（WBC）生成发挥调节作用。最后，miR - 223可作为CLL患者的预后因素；miR - 125a基于与BCl - 2的反向联系可能有助于评估治疗方法。
