Hodinka R L, Gilligan P H
Clinical Microbiology-Immunology Laboratories, North Carolina Memorial Hospital, Chapel Hill.
J Clin Microbiol. 1988 Jan;26(1):47-9. doi: 10.1128/jcm.26.1.47-49.1988.
The utility of a rapid latex slide agglutination test (Campyslide; BBL Microbiology Systems, Cockeysville, Md.) in detecting selected Campylobacter spp. was evaluated and compared with that of conventional identification methods. Isolated colonies suggestive of Campylobacter spp. were tested directly from primary selective media after incubation at 42 degrees C under microaerophilic conditions. Stock cultures of Campylobacter jejuni (n = 27) and C. coli (n = 3) were correctly confirmed to the genus level by latex agglutination when tested in pure cultures or isolated from seeded human feces. A total of 50 fresh clinical isolates of Campylobacter spp. (45 C. jejuni and 5 C. coli) were examined, with complete agreement observed between the latex test and conventional methods. Of 173 non-Campylobacter isolates tested from primary plates, only 1 rough strain of Pseudomonas aeruginosa produced a false-positive result. Although the manufacturer recommends a 30-min antigen extraction, 1 or 5 min was found to be sufficient. Also, confirmation could be achieved within 24 h of inoculation of clinical specimens, 2 days earlier than with conventional methods.
评估了快速乳胶玻片凝集试验（Campyslide；BBL微生物系统公司，马里兰州科基斯维尔）在检测特定弯曲杆菌属细菌方面的效用，并与传统鉴定方法进行了比较。在微需氧条件下于42℃孵育后，直接从初代选择性培养基中对疑似弯曲杆菌属的分离菌落进行检测。空肠弯曲菌（n = 27）和大肠弯曲菌（n = 3）的储备培养物，在纯培养物中进行检测或从接种了菌的人粪便中分离出来时，通过乳胶凝集试验均能正确鉴定到属水平。共检查了50株弯曲杆菌属的新鲜临床分离株（45株空肠弯曲菌和5株大肠弯曲菌），乳胶试验与传统方法结果完全一致。在从初代平板上检测的173株非弯曲杆菌分离株中，只有1株铜绿假单胞菌粗糙菌株产生了假阳性结果。尽管制造商推荐进行30分钟的抗原提取，但发现1分钟或5分钟就足够了。此外，在接种临床标本后24小时内即可完成鉴定，比传统方法提前2天。
