Ostrowski Nicole, Lee Boeun, Hong Daeho, Enick P Nathan, Roy Abhijit, Kumta Prashant N
Swanson School of Engineering, Department of Bioengineering, University of Pittsburgh, 3700 O'Hara Street, Pittsburgh, Pennsylvania 15261, United States.
ACS Biomater Sci Eng. 2015 Jan 12;1(1):52-63. doi: 10.1021/ab500073c. Epub 2014 Dec 12.
Magnesium phosphate implants may be used for bone void filling applications, potentially replacing traditionally studied bioceramics, which suffer from limited resorption and inferior mechanical properties compared to natural bone. In this study, amorphous and crystalline trimagnesium phosphates were synthesized and characterized utilizing a variety of analytical methods. In vitro solubility and cytotoxicity of the corresponding amorphous and crystalline phosphates were also analyzed. Amorphous magnesium phosphate was shown to be more soluble than the crystalline counterpart in vitro while inducing mineralization of an amorphous phosphate phase mimicking hydroxyapatite-type characteristic morphology on the substrate surface. The rapid mineralization of the amorphous magnesium phosphate was found to promote the proliferation and differentiation of osteoblast-like cells in comparison to the crystalline phase. Both magnesium phosphates hindered the differentiation of monocytes into osteoclasts. The combined effects of the spontaneous serum-mediated apatite-like mineralization, increased osteoblast differentiation and suspended osteoclast formation indicate that the amorphous magnesium phosphates may be promising bioactive materials for bone void repair applications.
磷酸镁植入物可用于骨缺损填充应用，有可能替代传统研究的生物陶瓷，与天然骨相比，传统生物陶瓷存在吸收有限和机械性能较差的问题。在本研究中，利用多种分析方法合成并表征了无定形和结晶态的磷酸三镁。还分析了相应无定形和结晶态磷酸盐的体外溶解性和细胞毒性。结果表明，无定形磷酸镁在体外比结晶态磷酸镁更易溶解，同时能诱导无定形磷酸盐相矿化，在底物表面模拟羟基磷灰石型特征形态。与结晶态相比，发现无定形磷酸镁的快速矿化促进了成骨样细胞的增殖和分化。两种磷酸镁都阻碍单核细胞向破骨细胞的分化。自发血清介导的类磷灰石矿化、成骨细胞分化增加和破骨细胞形成受阻的综合作用表明，无定形磷酸镁可能是用于骨缺损修复应用的有前景的生物活性材料。
