To Kimberly T, St Mary Lindsey, Wooley Allyson H, Wilbanks Mitchell S, Bednar Anthony J, Perkins Edward J, Truong Lisa, Tanguay Robyn L, Garcia-Reyero Natàlia
Environmental Laboratory, US Army Engineer Research & Development Center, Vicksburg, MS 39180, USA.
The Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, Oregon State University, Corvallis, OR 97333, USA.
Toxics. 2021 Jan 10;9(1):9. doi: 10.3390/toxics9010009.
Solvent Violet 47 (SV47) and Disperse Blue 14 (DB14) are two anthraquinone dyes that were previously used in different formulations for the production of violet-colored smoke. Both dyes have shown potential for toxicity; however, there is no comprehensive understanding of their effects. Zebrafish embryos were exposed to SV47 or DB14 from 6 to 120 h post fertilization (hpf) to assess the dyes' potential adverse effects on developing embryos. The potential ability of both dyes to cross the blood-brain barrier was also assessed. At concentrations between 0.55 and 5.23 mg/L, SV47 showed a dose-dependent increase in mortality, jaw malformation, axis curvature, and edemas. At concentrations between 0.15 and 7.54 mg/L, DB14 did not have this same dose-dependence but had similar morphological outcomes at the highest doses. Nevertheless, while SV47 showed significant mortality from 4.20 mg/L, there was no significant mortality on embryos exposed to DB14. Regardless, decreased locomotor movement was observed at all concentrations of DB14, suggesting an adverse neurodevelopmental effect. Overall, our results showed that at similar concentrations, SV47 and DB14 caused different types of phenotypic effects in zebrafish embryos.
溶剂紫47（SV47）和分散蓝14（DB14）是两种蒽醌染料，以前曾用于不同配方中以生产紫色烟雾。两种染料均显示出潜在的毒性；然而，对它们的影响尚无全面的了解。将斑马鱼胚胎在受精后6至120小时（hpf）暴露于SV47或DB14，以评估这些染料对发育中胚胎的潜在不利影响。还评估了两种染料穿过血脑屏障的潜在能力。在0.55至5.23 mg/L的浓度下，SV47显示出死亡率、颌骨畸形、轴弯曲和水肿呈剂量依赖性增加。在0.15至7.54 mg/L的浓度下，DB14没有这种相同的剂量依赖性，但在最高剂量下有类似的形态学结果。然而，虽然SV47在4.20 mg/L时显示出显著的死亡率，但暴露于DB14的胚胎没有显著的死亡率。无论如何，在所有DB14浓度下均观察到运动活动减少，表明存在不良的神经发育影响。总体而言，我们的结果表明，在相似浓度下，SV47和DB14在斑马鱼胚胎中引起了不同类型的表型效应。
