Barbadoro Pamela, Bencardino Daniela, Carloni Elisa, Omiccioli Enrica, Ponzio Elisa, Micheletti Rebecca, Acquaviva Giorgia, Luciani Aurora, Masucci Annamaria, Pocognoli Antonella, Orecchioni Francesca, D'Errico Marcello Mario, Magnani Mauro, Andreoni Francesca
Department of Biomedical Science and Public Health, Università Politecnica delle Marche, 60122 Ancona, Italy.
SOD Igiene Ospedaliera-AOU Ancona Associated Hospitals, 60126 Ancona, Italy.
Antibiotics (Basel). 2021 Jan 10;10(1):61. doi: 10.3390/antibiotics10010061.
The emerging spread of carbapenemase-producing Enterobacterales (CPE) strains, in particular, and , has become a significant threat to hospitalized patients. Carbapenemase genes are frequently located on plasmids than can be exchanged among clonal strains, increasing the antibiotic resistance rate. The aim of this study was to determine the prevalence of CPE in patients upon their admission and to analyze selected associated factors. An investigation of the antibiotic resistance and genetic features of circulating CPE was carried out. Phenotypic tests and molecular typing were performed on 48 carbapenemase-producing strains of and collected from rectal swabs of adult patients. Carbapenem-resistance was confirmed by PCR detection of resistance genes. All strains were analyzed by PCR-based replicon typing (PBRT) and multilocus sequence typing (MLST) was performed on a representative isolate of each PBRT profile. More than 50% of the strains were found to be multidrug-resistant, and the gene was detected in all the isolates with the exception of an strain. A multireplicon status was observed, and the most prevalent profile was FIIK, FIB KQ (33%). MLST analysis revealed the prevalence of sequence type 512 (ST512). This study highlights the importance of screening patients upon their admission to limit the spread of CRE in hospitals.
产碳青霉烯酶肠杆菌目细菌（CPE）菌株的不断传播，尤其是[具体菌株1]和[具体菌株2]，已对住院患者构成重大威胁。碳青霉烯酶基因通常位于质粒上，可在克隆菌株间交换，从而提高了抗生素耐药率。本研究的目的是确定患者入院时CPE的流行情况，并分析相关因素。对循环CPE的抗生素耐药性和基因特征进行了调查。对从成年患者直肠拭子中收集的48株产碳青霉烯酶的[具体菌株1]和[具体菌株2]进行了表型试验和分子分型。通过PCR检测耐药基因来确认碳青霉烯耐药性。所有菌株均通过基于PCR的复制子分型（PBRT）进行分析，并对每个PBRT谱型的代表性分离株进行多位点序列分型（MLST）。发现超过50%的菌株具有多重耐药性，除一株[具体菌株]外，所有分离株均检测到[具体基因]。观察到多复制子状态，最常见的谱型是FIIK、FIB KQ（33%）。MLST分析显示序列型512（ST512）的流行情况。本研究强调了患者入院时进行筛查以限制医院中耐碳青霉烯肠杆菌传播的重要性。
