Xu Hao, Miao Xin, Li Xin, Chen Haofei, Zhang Bo, Zhou Wence
The Second Department of General Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu, China.
The First Clinical Medical School of Lanzhou University, Lanzhou, Gansu, China.
Cancer Cell Int. 2021 Jan 12;21(1):51. doi: 10.1186/s12935-020-01715-9.
It has been reported that the lncRNA SNHG16 has significantly increased expression in pancreatic adenocarcinoma (PC). However, the functions and mechanisms of SNHG16 are not clear. The aim of this study was to explore the effects of SNHG16 on PC.
qRT-PCR analysis was applied to detect the expression levels of SNHG16, miR-302b-3p and SLC2A4 in PC tissues and cells. CCK8 and EdU assays were used to evaluate the proliferation of PC cells. Transwell assays were used to assess PC cell migration and invasion. Apoptosis was evaluated by flow cytometry, and the expression of apoptosis-related proteins (including Bax, Bcl-2, cleaved caspase-3 and cleaved caspase-9) was tested by western blotting. The interactions between miR-302b-3p and SNHG16 or miR-302b-3p and the 3'UTR of SLC2A4 mRNA were clarified by a dual luciferase reporter assay and RNA immunoprecipitation.
SNHG16 expression was significantly elevated in PC tissues and cell lines and was associated with poor prognosis of PC patients. Knockdown of SNHG16 reduced PC cell proliferation, migration and invasion. SNHG16 acted as a sponge to regulate miR-302b-3p expression in PC cells. In addition, miR-302b-3p targeted SLC2A4 directly.
SNHG16 promoted the progression of PC via the miR-302b-3p/SLC2A4 axis and was expected to be a potential target for the early diagnosis and treatment of PC.
据报道，长链非编码RNA SNHG16在胰腺腺癌（PC）中表达显著增加。然而，SNHG16的功能和机制尚不清楚。本研究旨在探讨SNHG16对PC的影响。
应用qRT-PCR分析检测PC组织和细胞中SNHG16、miR-302b-3p和SLC2A4的表达水平。采用CCK8和EdU检测评估PC细胞的增殖。采用Transwell检测评估PC细胞的迁移和侵袭。通过流式细胞术评估细胞凋亡，并通过蛋白质印迹法检测凋亡相关蛋白（包括Bax、Bcl-2、裂解的caspase-3和裂解的caspase-9）的表达。通过双荧光素酶报告基因检测和RNA免疫沉淀明确miR-302b-3p与SNHG16或miR-302b-3p与SLC2A4 mRNA的3'UTR之间的相互作用。
SNHG16在PC组织和细胞系中表达显著升高，且与PC患者的不良预后相关。敲低SNHG16可降低PC细胞的增殖、迁移和侵袭。SNHG16作为海绵调节PC细胞中miR-302b-3p的表达。此外，miR-302b-3p直接靶向SLC2A4。
SNHG16通过miR-302b-3p/SLC2A4轴促进PC进展，有望成为PC早期诊断和治疗的潜在靶点。
