Noh Miyeon, Choi Young Hwan, An Young-Hyeon, Tahk Dongha, Cho Sungwoo, Yoon Jung Won, Jeon Noo Li, Park Tai Hyun, Kim Jaeho, Hwang Nathaniel S
Interdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Republic of Korea.
School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 08826, Republic of Korea.
ACS Biomater Sci Eng. 2019 Aug 12;5(8):3909-3921. doi: 10.1021/acsbiomaterials.8b01307. Epub 2019 Feb 20.
Endothelial progenitor cells (EPCs) can induce a pro-angiogenic response during tissue repair. Recently, EPC transplantations have been widely investigated in wound healing applications. To maximize the healing efficacy by EPCs, a unique scaffold design that allows cell retention and function would be desirable for delivery. Herein, we fabricated an alginate/poly-l-ornithine/gelatin (alginate-PLO-gelatin) hydrogel sheet with a groove pattern for use as a cell delivery platform. In addition, we demonstrate the topographical modification of the hydrogel sheet surface with a groove pattern to modulate cell proliferation, alignment, and elongation. We report that the patterned substrate prompted morphological changes of endothelial cells, increased cell-cell interaction, and resulted in the active secretion of growth factors such as PDGF-BB. Additionally, we incorporated magnetic nanoparticles (MNPs) into the patterned hydrogel sheet for the magnetic field-induced transfer of cell-seeded hydrogel sheets. As a result, enhanced wound healing was observed via efficient transplantation of the EPCs with an MNP-embedded patterned hydrogel sheet (MPS). Finally, enhanced vascularization and dermal wound repair were observed with EPC seeded MPS.
内皮祖细胞（EPCs）在组织修复过程中可诱导促血管生成反应。近来，EPC移植已在伤口愈合应用中得到广泛研究。为了通过EPCs实现最大愈合功效，一种能够实现细胞留存和功能的独特支架设计对于递送而言将是理想的。在此，我们制备了具有凹槽图案的藻酸盐/聚-L-鸟氨酸/明胶（藻酸盐-PLO-明胶）水凝胶片用作细胞递送平台。此外，我们展示了通过凹槽图案对水凝胶片表面进行形貌修饰以调节细胞增殖、排列和伸长。我们报告称，图案化基质促使内皮细胞发生形态变化，增加了细胞间相互作用，并导致诸如血小板衍生生长因子-BB（PDGF-BB）等生长因子的活性分泌。此外，我们将磁性纳米颗粒（MNPs）掺入图案化水凝胶片中，用于磁场诱导接种细胞的水凝胶片转移。结果，通过用嵌入MNP的图案化水凝胶片（MPS）有效移植EPCs，观察到伤口愈合得到增强。最后，接种EPC的MPS观察到血管生成增强和皮肤伤口修复。
