Wang Li-Sheng, Gupta Akash, Duncan Bradley, Ramanathan Rajesh, Yazdani Mahdieh, Rotello Vincent M
Department of Chemistry, University of Massachusetts-Amherst, 710 N. Pleasant St., Amherst, Massachusetts 01003, United States.
Ian Potter NanoBioSensing Facility and NanoBiotechnology Research Laboratory, School of Applied Sciences, RMIT University, 124 La Trobe Street, Melbourne, Victoria 3000, Australia.
ACS Biomater Sci Eng. 2016 Nov 14;2(11):1862-1866. doi: 10.1021/acsbiomaterials.6b00464. Epub 2016 Sep 7.
Bacteria attach to the surfaces of medical devices and implants, resulting in life-threatening infections. Nonfouling coatings can be used to prevent adhesion of bacteria on the surface, while biocidal coatings kill the microbes. Combining nonfouling and biocidal properties can yield highly effective antimicrobial coatings. We demonstrate here a nanoimprint lithography (NIL)-based method to generate antibacterial coatings that both resist bacterial attachment and kill bacteria. In this strategy nanoimprint lithography was used to create water-stable films of bovine serum albumin (BSA) that are nonadhesive toward bacteria because of their negative/zwitterionic surface potential. Biocidal activity was then imparted through chlorination of cysteine sulfurs, providing slow release of chlorine and potent antimicrobial activity against pathogenic bacteria.
细菌附着在医疗设备和植入物表面，会导致危及生命的感染。防污涂层可用于防止细菌在表面附着，而杀菌涂层则能杀死微生物。将防污和杀菌特性相结合可产生高效的抗菌涂层。我们在此展示一种基于纳米压印光刻（NIL）的方法来制备抗菌涂层，该涂层既能抵抗细菌附着又能杀死细菌。在这一策略中，纳米压印光刻被用于制造牛血清白蛋白（BSA）的水稳定薄膜，由于其负电荷/两性离子表面电位，该薄膜对细菌无粘附性。然后通过对半胱氨酸硫进行氯化赋予杀菌活性，从而实现氯的缓慢释放以及对病原菌的强效抗菌活性。
