Genetics and Bioinformatics Department, Dasman Diabetes Institute, Kuwait City, Kuwait.
Kuwait Identification DNA Laboratory, General Department of Criminal Evidence, Ministry of Interior, Kuwait City, Kuwait.
Sci Rep. 2021 Jan 13;11(1):1091. doi: 10.1038/s41598-020-80040-7.
Obesity, a major risk factor for metabolic disorders, is highly prevalent in Qatari population. Maternal transmission of obesity traits can be significant; for example, X haplogroup is known to be associated with lower BMI and body fat mass in Northern Europeans and T haplogroup which is a sister haplogroup of J is known to be associated with obesity in Caucasian subjects from Austria and Southern Italy. We aimed to delineate the mitochondrial haplogroups and variants associated with obesity in Qatari population. Mitochondrial genomes of 864 Qatari individuals were extracted from whole exome sequencing data with an average coverage of 77X. We distributed the participants into 2 sub-cohorts: obese (BMI ≥ 30) and non-obese (BMI < 30); the mean value of BMI from these two groups were 36.5 ± 5.7 and 26.5 ± 2.6, respectively. Mitochondrial haplogroup profiling followed by uni- and multivariant association tests adjusted for covariates were performed. Qatari individuals with mitochondrial haplogroup J had an increased (twofold) risk of obesity (odds ratio [OR] 1.925; 95% CI 1.234-3.002; P = 0.0038; the Bonferroni adjusted P value threshold is 0.0041), whereas the individuals with haplogroup X were at low risk of obesity (OR 0.387; 95% CI 0.175-0.857; P = 0.019). Further, a set of 38 mitochondrial variants were found to be associated (at P ≤ 0.05) with obesity in models adjusted for age, sex and haplogroup.
肥胖是代谢紊乱的一个主要危险因素，在卡塔尔人口中高度流行。肥胖特征的母体传递可能是显著的；例如，X 单倍群已知与北欧人的 BMI 和体脂肪量较低相关，而 T 单倍群是 J 单倍群的姊妹单倍群，已知与奥地利和意大利南部的白种人肥胖有关。我们旨在描绘与卡塔尔人口肥胖相关的线粒体单倍群和变体。从外显子组测序数据中提取了 864 名卡塔尔个体的线粒体基因组，平均覆盖度为 77X。我们将参与者分为 2 个亚组：肥胖（BMI≥30）和非肥胖（BMI<30）；这两组的 BMI 平均值分别为 36.5±5.7 和 26.5±2.6。进行了线粒体单倍群分析，然后进行了单变量和多变量关联测试，并调整了协变量。具有线粒体单倍群 J 的卡塔尔个体肥胖的风险增加（两倍）（优势比[OR]1.925；95%置信区间[CI]1.234-3.002；P=0.0038；Bonferroni 调整后的 P 值阈值为 0.0041），而具有线粒体单倍群 X 的个体肥胖的风险较低（OR 0.387；95%CI 0.175-0.857；P=0.019）。此外，在调整年龄、性别和单倍群的模型中，发现一组 38 个线粒体变体与肥胖相关（在 P≤0.05 时）。
