Department of Gastroenterology, Tsukaguchi Hospital, 6-8-1 Minamitsukaguchi-cho, Amagasaki, Hyogo, 661-0012, Japan.
Yui-Nozomi Hospital, 95 Fushimido, Tondabayashi, Osaka, 584-0055, Japan.
Sci Rep. 2021 Jan 13;11(1):1072. doi: 10.1038/s41598-020-80443-6.
Disinfectants have different efficacies depending on their use and the target microorganism. This study aimed to evaluate the efficacy and safety of our new nonalcoholic disinfectant, which consists mainly of metal ions. According to the 17th revised Japanese Pharmacopoeia and ASTM international E1052 method, the bactericidal and virucidal efficacy of this new disinfectant against 13 microorganisms was evaluated by the in vitro quantitative suspension test. Additionally, the disinfectant cytotoxicity against multiple cell lines was examined. Then, a safety test using a human open patch test was performed with 26 healthy volunteers. This disinfectant showed strong bactericidal and virucidal activities: all microorganisms except enterovirus were inactivated very quickly. The infectivity of 12 microbial strains was eliminated within 5 min of disinfectant exposure. Additionally, this disinfectant showed little acute cytotoxicity in vitro. All volunteers were negative in the human open patch test. Our new disinfectant has a broad spectrum of microbial targets, is safe for human skin, and demonstrates no cytotoxicity. This disinfectant could prevent common microbial infections.
消毒剂的功效因用途和目标微生物的不同而有所差异。本研究旨在评估我们新型的主要成分为金属离子的非酒精类消毒剂的功效和安全性。根据第十七版日本药局方和 ASTM 国际 E1052 方法，通过体外定量悬液试验评估了这种新型消毒剂对 13 种微生物的杀菌和杀病毒功效。此外，还检测了消毒剂对多种细胞系的细胞毒性。然后，对 26 名健康志愿者进行了人体开放性斑贴试验的安全性测试。这种消毒剂具有很强的杀菌和杀病毒活性：除肠道病毒外，所有微生物都很快被灭活。12 种微生物菌株的感染性在接触消毒剂后 5 分钟内被消除。此外，这种消毒剂在体外的急性细胞毒性很小。所有人在人体开放性斑贴试验中均呈阴性。我们的新型消毒剂具有广谱的微生物靶标，对人体皮肤安全，且无细胞毒性。这种消毒剂可以预防常见的微生物感染。
