Aranda-Anzaldo A, Viza D, Busnel R G
C.N.R.S., Laboratoire d'Immunobiologie, Faculté de Médecine, Paris, France.
J Virol Methods. 1992 Apr;37(1):71-81. doi: 10.1016/0166-0934(92)90021-5.
Most chemicals with potential virucidal activity are extremely cytotoxic even at very small concentrations, thus introducing a number of technical problems and uncertainties in the evaluation of the net virucidal effect. In the present study, an attempt was made to confirm the reported virucidal activity of certain well-known chemicals and a number of new compounds were investigated. The results suggest that HIV inactivation is dependent on the viral concentration, the time of incubation in presence of the putative disinfectant and the degree of virucidal activity of the latter. The data illustrate methodological problems arising from residual cytotoxicity of the chemical which may mask or mimic the presence of a true virucidal activity and lead to erroneous conclusions. Alcohol, the most commonly used disinfectant, was found to be ineffective for high viral concentrations, whilst sodium hypochlorite was the most efficient.
大多数具有潜在杀病毒活性的化学物质即使在极低浓度下也具有极强的细胞毒性，因此在评估净杀病毒效果时会带来一系列技术问题和不确定性。在本研究中，我们试图确认某些知名化学物质已报道的杀病毒活性，并对一些新化合物进行了研究。结果表明，HIV失活取决于病毒浓度、在假定消毒剂存在下的孵育时间以及后者的杀病毒活性程度。数据说明了化学物质残留细胞毒性所引发的方法学问题，这种毒性可能掩盖或模拟真正的杀病毒活性的存在，并导致错误的结论。酒精是最常用的消毒剂，发现其对高病毒浓度无效，而次氯酸钠是最有效的。
