Yu Xiaoling, Jiang Wenqian
Department of Infectious Diseases, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, People's Republic of China.
Infect Drug Resist. 2021 Jan 6;14:11-16. doi: 10.2147/IDR.S282190. eCollection 2021.
Non-tuberculous mycobacteria are conditional pathogens that can cause many diseases, among which pulmonary infections are the most common (65-90%). and are non-tuberculous mycobacteria most often associated with lung diseases. Mass spectrometry diagnostic techniques were not effective in complex infection. We report a case of and complex causing severe pneumonia in an adult with HIV. Our group developed a novel molecular-based method to identify species. Novel techniques such as molecular cloning which we have described here can make up for the inability of matrix-assisted laser desorption ionization-time of flight mass spectrometry to distinguish the multiple microorganisms present, and may add to the diagnostic toolkit and increase the accuracy and rapidity of diagnosis in the future.
非结核分枝杆菌是可导致多种疾病的条件致病菌，其中肺部感染最为常见（65%-90%），且是最常与肺部疾病相关的非结核分枝杆菌。质谱诊断技术在复杂感染中无效。我们报告1例由鸟分枝杆菌和胞内分枝杆菌复合菌引起的成年HIV患者严重肺炎病例。我们团队开发了一种基于分子的新方法来鉴定分枝杆菌菌种。我们在此描述的诸如分子克隆等新技术可弥补基质辅助激光解吸电离飞行时间质谱无法区分多种存在的微生物的不足，并可能在未来增加诊断工具并提高诊断的准确性和快速性。
