and Complex Causing Severe Pneumonia in a Patient with HIV Identified by a Novel Molecular-Based Method.

Non-tuberculous mycobacteria are conditional pathogens that can cause many diseases, among which pulmonary infections are the most common (65-90%). and are non-tuberculous mycobacteria most often associated with lung diseases. Mass spectrometry diagnostic techniques were not effective in complex infection. We report a case of and complex causing severe pneumonia in an adult with HIV. Our group developed a novel molecular-based method to identify species. Novel techniques such as molecular cloning which we have described here can make up for the inability of matrix-assisted laser desorption ionization-time of flight mass spectrometry to distinguish the multiple microorganisms present, and may add to the diagnostic toolkit and increase the accuracy and rapidity of diagnosis in the future.