Pięt Mateusz, Zając A, Paduch R, Jaszek M, Frant M, Stefaniuk D, Matuszewska A, Grzywnowicz K
Department of Virology and Immunology, Maria Curie-Sklodowska University, Akademicka Str. 19, 20-033 Lublin, Poland.
Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
3 Biotech. 2021 Jan;11(1):5. doi: 10.1007/s13205-020-02591-w. Epub 2021 Jan 1.
The biochemical properties and anti-tumorigenic activity of (CU) and (PS) towards colon cancer cells and the effect of supplementation of the fungal culture medium with cow milk on these activities were examined. CU1-II and PS4-II exhibited anticancer properties through various mechanisms. The extracts at the 200 µg/mL concentration significantly decreased the viability of HT-29 and SW948 cells. They also exhibited pro-apoptotic properties towards the cancer cell lines (HT-29, LS 180, and SW948). Furthermore, culturing the studied fungi on milk-supplemented media may improve the pro-health properties of both milk and mushrooms. The extracts had a higher concentration of proteins, lower levels of free amino acids, and higher content of phenolic compounds than milk. They also exerted a free radical scavenging effect, which may be connected with the high activity of catalase and superoxide dismutase. The tested extracts exhibited anticancer activity: grown on the medium without milk and grown on the medium with milk. The CU1-II and PS4-II extracts exhibited the strongest anticancer properties; however, PS4-II exerted a milder effect on normal CCD 841 CoTr cells than CU1-II. CU3-II exerted the mildest effect among all extracts on both normal and cancer cells.
研究了（CU）和（PS）对结肠癌细胞的生化特性和抗肿瘤活性，以及在真菌培养基中添加牛奶对这些活性的影响。CU1-II和PS4-II通过多种机制表现出抗癌特性。浓度为200µg/mL的提取物显著降低了HT-29和SW948细胞的活力。它们还对癌细胞系（HT-29、LS 180和SW948）表现出促凋亡特性。此外，在添加牛奶的培养基上培养所研究的真菌可能会改善牛奶和蘑菇的有益健康特性。提取物比牛奶含有更高浓度的蛋白质、更低水平的游离氨基酸和更高含量的酚类化合物。它们还具有自由基清除作用，这可能与过氧化氢酶和超氧化物歧化酶的高活性有关。测试的提取物表现出抗癌活性：在无牛奶培养基上生长的和在含牛奶培养基上生长的。CU1-II和PS4-II提取物表现出最强的抗癌特性；然而，PS4-II对正常CCD 841 CoTr细胞的影响比CU1-II更温和。CU3-II在所有提取物中对正常细胞和癌细胞的影响最温和。
