Composting Research Group, Department of Chemical, Biological and Environmental Engineering. Escola d'Enginyeria, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193 Barcelona, Spain.
Composting Research Group, Department of Chemical, Biological and Environmental Engineering. Escola d'Enginyeria, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193 Barcelona, Spain.
Waste Manag. 2021 Feb 15;121:403-411. doi: 10.1016/j.wasman.2020.12.036. Epub 2021 Jan 11.
2-phenylethanol (2-PE) is a value-added compound widely used in industry due to its rose-like odor and antibacterial properties that can be bioproduced using wastes as raw materials. This study presents the valorization of nine agro-industrial wastes as potential substrates for 2-PE production using an isolated 2-PE producer Pichia kudriavzevii, and the solid-state fermentation (SSF) technology as an alternative approach. The assessed substrates comprised wastes of varied traits such that each of them provided different characteristics to the fermentation. Thus, by using a principal component analysis (PCA), it was possible to identify the most significant characteristics associated with the substrates affecting the 2-PE production. Results show that L-phenylalanine biotransformation was more efficient than de novo synthesis for producing 2-PE. Besides, from the evaluated set, the maximum 2-PE production was achieved with red apple pomace, reaching 1.7 and 25.2 mg per gram of used waste through de novo and L-phenylalanine biotransformation, respectively. In that scenario, volumetric productivity and precursor yield were 39.6 mg Lh and 0.69 g per gram of L-phenylalanine added respectively. From the PCA, it was identified that the reducing sugars content of the substrate, the air-filled porosity of the bed and the L-phenylalanine availability were the most critical parameters (associated with the substrates) influencing the microbial activity and 2-PE production. These results suggest that the desirable traits a solid media needs for promoting 2-PE production via SSF could be reached by using a combination of wastes in a synergistic approach.
2-苯乙醇（2-PE）是一种附加值化合物，由于其玫瑰般的气味和抗菌性能，广泛应用于工业领域，可以利用废物作为原料进行生物生产。本研究利用分离得到的 2-PE 生产菌毕赤酵母（Pichia kudriavzevii），以及固态发酵（SSF）技术，将 9 种农业工业废物作为潜在的 2-PE 生产底物进行了增值利用。评估的底物包括具有不同特性的废物，它们为发酵提供了不同的特性。因此，通过主成分分析（PCA），可以确定与影响 2-PE 生产的底物相关的最显著特征。结果表明，L-苯丙氨酸生物转化比从头合成更有利于生产 2-PE。此外，在所评估的废物中，红苹果渣的 2-PE 产量最高，通过从头合成和 L-苯丙氨酸生物转化，每克使用的废物分别达到 1.7 和 25.2mg。在这种情况下，体积生产率和前体产率分别为 39.6mg/Lh 和 0.69g 每克添加的 L-苯丙氨酸。通过 PCA 确定，底物的还原糖含量、床的充气孔隙率和 L-苯丙氨酸的可用性是影响微生物活性和 2-PE 生产的最关键参数（与底物相关）。这些结果表明，通过 SSF 促进 2-PE 生产，固态培养基需要的理想特性可以通过协同利用废物来实现。
