Agarwal Sankalp, Riffault Mathieu, Hoey David, Duffy Brendan, Curtin James, Jaiswal Swarna
Centre for Research in Engineering and Surface Technology, FOCAS Institute, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland.
School of Food Science and Environmental Health, Dublin Institute of Technology, Cathal Brugha Street, Dublin 1, Ireland.
ACS Biomater Sci Eng. 2017 Dec 11;3(12):3244-3253. doi: 10.1021/acsbiomaterials.7b00527. Epub 2017 Nov 16.
This study presents the covalent grafting of a hyaluronic acid-lysozyme (HA-LZ) composite onto corrosion-resistant silane-coated AZ31 Mg alloy via EDC-NHS coupling reactions. The HA-LZ composite coatings created a smooth and hydrophilic surface with the increased concentration of functional lysozyme complexed to the hyaluronic acid. This was confirmed by the measurement of AFM, water contact angle, and quantification of hyaluronic acid and lysozyme. The colonization of on HA-LZ composite-coated substrates was significantly reduced as compared to the hyaluronic acid, lysozyme coated and uncoated AZ31 controls. Such activity is due to the enhanced antibacterial activity of lysozyme component as observed from the spread plate assay, propidium iodide staining, and scanning electron microscopy. Furthermore, morphology of the osteoblast cells, alkaline phosphatase activity and DNA quantification studies demonstrated the improved biocompatibility and osteoinductive properties of HA-LZ-coated substrates. This was verified by comparing with the lysozyme coated and uncoated AZ31 substrates in terms of cell adhesion, proliferation, and differentiation of osteoblastic cells. Therefore, such multifunctional composite coatings with antibacterial and osteoinductive properties are promising can be potentially used for the surface modifications of orthopedic implants.
本研究通过EDC-NHS偶联反应将透明质酸-溶菌酶（HA-LZ）复合物共价接枝到耐腐蚀硅烷涂层的AZ31镁合金上。HA-LZ复合涂层形成了光滑且亲水的表面，与透明质酸复合的功能性溶菌酶浓度增加。这通过原子力显微镜（AFM）测量、水接触角以及透明质酸和溶菌酶的定量得以证实。与透明质酸、溶菌酶涂层以及未涂层的AZ31对照相比，HA-LZ复合涂层底物上的细菌定植显著减少。这种活性归因于从平板扩散试验、碘化丙啶染色和扫描电子显微镜观察到的溶菌酶成分增强的抗菌活性。此外，成骨细胞的形态、碱性磷酸酶活性和DNA定量研究证明了HA-LZ涂层底物具有改善的生物相容性和骨诱导特性。通过在成骨细胞的细胞粘附、增殖和分化方面与溶菌酶涂层以及未涂层的AZ31底物进行比较，证实了这一点。因此，这种具有抗菌和骨诱导特性的多功能复合涂层很有前景，可潜在地用于骨科植入物的表面改性。
