Faculty of Sciences Semlalia, Cadi Ayyad University, Avenue Prince Moulay Abdellah, BP 2390, 40000 Marrakesh, Morocco.
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Japan.
Molecules. 2021 Jan 12;26(2):371. doi: 10.3390/molecules26020371.
The use of natural products for the regulation of skin pigmentation is gaining popularity. In the present study, we evaluated the effect of argan leaves extract (ALE) on melanogenesis in B16 melanoma cells, determined its antioxidant activity, then quantified and identified its phenolic components. B16 cells were treated with various concentrations of ALE, then the cell viability and proliferation were assessed using MTT assay while the melanin content was determined using spectrophotometric methods. The expression level of tyrosinase (TYR), tyrosinase related protein-1 (TRP-1) and dopachrome tautomerase (DCT) was evaluated by Western blotting. The antioxidant activity of ALE was investigated using four different assays while UPLC-ESI-HRMS analysis was used to characterize the ALE phenolic profile. Fourteen phenolic compounds were identified, of which six are reported for the first time to be present in ALE. ALE treatment increases the melanin content of B16 cells in a dose-dependent manner without cytotoxicity. This was revealed by the observed ALE-increased expression level of TYR, DCT, and TRP-1. These bioactivities may be mainly attributed to its high flavonoids content. Argan leaves have the potential for use as a treatment for hypopigmentation disorders and as a bioactive component of cosmetic products that aim to increase pigmentation.
天然产物在调节皮肤色素沉着方面的应用越来越受到关注。本研究评估了阿甘树叶提取物（ALE）对 B16 黑素瘤细胞中黑色素生成的影响，测定了其抗氧化活性，然后对其酚类成分进行了定量和鉴定。用不同浓度的 ALE 处理 B16 细胞，然后通过 MTT 法评估细胞活力和增殖，分光光度法测定黑色素含量。通过 Western blot 法评估酪氨酸酶（TYR）、酪氨酸酶相关蛋白-1（TRP-1）和多巴色素互变异构酶（DCT）的表达水平。采用四种不同的测定方法研究 ALE 的抗氧化活性，并用 UPLC-ESI-HRMS 分析对 ALE 的酚类成分进行了表征。鉴定出 14 种酚类化合物，其中 6 种是首次在 ALE 中发现。ALE 处理以剂量依赖的方式增加 B16 细胞中的黑色素含量，而没有细胞毒性。这是通过观察到 ALE 增加 TYR、DCT 和 TRP-1 的表达水平来揭示的。这些生物活性可能主要归因于其高黄酮含量。阿甘树叶具有作为治疗色素减退性疾病的用途的潜力，并且可以作为旨在增加色素沉着的化妆品产品的生物活性成分。
