Natić Maja M, Dabić Dragana Č, Papetti Adele, Fotirić Akšić Milica M, Ognjanov Vladislav, Ljubojević Mirjana, Tešić Živoslav Lj
Faculty of Chemistry, University of Belgrade, P. O. Box 51, 11158 Belgrade, Serbia.
Innovation Centre, Faculty of Chemistry Ltd., University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
Food Chem. 2015 Mar 15;171:128-36. doi: 10.1016/j.foodchem.2014.08.101. Epub 2014 Sep 6.
In this study, the polyphenolic profile of 11 Morus alba fruits grown in the Vojvodina region was investigated. Ultra high performance liquid chromatography (UHPLC) coupled with Linear Trap Quadrupole and OrbiTrap mass analyzer, and UHPLC coupled with a diode array detector and a triple-quadrupole mass spectrometer were used for the identification and quantification of the polyphenols, respectively. A total of 14 hydroxycinnamic acid esters, 13 flavonol glycosides, and 14 anthocyanins were identified in the extracts with different distributions and contents according to the sampling. The total phenolic content ranged from 43.84 to 326.29 mg GAE/100g frozen fruit. The radical scavenging capacity (50.18-86.79%), metal chelating ability (0.21-8.15%), ferric ion reducing power (0.03-38.45 μM ascorbic acid) and superoxide anion radical scavenging activity (16.53-62.83%) were assessed. The findings indicated that mulberry polyphenolics may act as potent superoxide anion radical scavengers and reducing agents.
在本研究中，对伏伊伏丁那地区种植的11种桑椹果实的多酚谱进行了研究。分别采用超高效液相色谱（UHPLC）联用线性离子阱四极杆和轨道阱质谱仪，以及UHPLC联用二极管阵列检测器和三重四极杆质谱仪对多酚进行鉴定和定量。根据采样，在提取物中总共鉴定出14种羟基肉桂酸酯、13种黄酮醇苷和14种花青素，它们具有不同的分布和含量。总酚含量范围为43.84至326.29 mg GAE/100g冷冻果实。评估了自由基清除能力（50.18 - 86.79%）、金属螯合能力（0.21 - 8.15%）、铁离子还原能力（0.03 - 38.45 μM抗坏血酸）和超氧阴离子自由基清除活性（16.53 - 62.83%）。研究结果表明，桑椹多酚可能作为有效的超氧阴离子自由基清除剂和还原剂。
