Zhang Rong, Jiang Weitao, Liu Xin, Duan Yanan, Xiang Li, Wang Yanfang, Jiang Yuanmao, Shen Xiang, Chen Xuesen, Yin Chengmiao, Mao Zhiquan
State Key Laboratory of Crop Biology/College of Horticultural Science and Engineering, Shandong Agricultural University, Daizong Road No.61, Tai'an, 271018, Shandong, China.
State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
Proteome Sci. 2021 Jan 14;19(1):2. doi: 10.1186/s12953-021-00170-2.
Apple replant disease (ARD) has been reported from all major fruit-growing regions of the world, and is often caused by biotic factors (pathogen fungi) and abiotic factors (phenolic compounds). In order to clarify the proteomic differences of Fusarium moniliforme under the action of phloridzin, and to explore the potential mechanism of F. moniliforme as the pathogen of ARD, the role of Fusarium spp. in ARD was further clarified.
In this paper, the quantitative proteomics method iTRAQ analysis technology was used to analyze the proteomic differences of F. moniliforme before and after phloridzin treatment. The differentially expressed protein was validated by qRT-PCR analysis.
A total of 4535 proteins were detected, and 293 proteins were found with more than 1.2 times (P< 0.05) differences. In-depth data analysis revealed that 59 proteins were found with more than 1.5 times (P< 0.05) differences, and most proteins were consistent with the result of qRT-PCR. Differentially expressed proteins were influenced a variety of cellular processes, particularly metabolic processes. Among these metabolic pathways, a total of 8 significantly enriched KEGG pathways were identified with at least 2 affiliated proteins with different abundance in conidia and mycelium. Functional pathway analysis indicated that up-regulated proteins were mainly distributed in amino sugar, nucleotide sugar metabolism, glycolysis/ gluconeogenesis and phagosome pathways.
This study is the first to perform quantitative proteomic investigation by iTRAQ labeling and LC-MS/MS to identify differentially expressed proteins in F. moniliforme under phloridzin conditions. The results confirmed that F. moniliforme presented a unique protein profile that indicated the adaptive mechanisms of this species to phloridzin environments. The results deepened our understanding of the proteome in F. moniliforme in response to phloridzin inducers and provide a basis for further exploration for improving the efficiency of the fungi as biocontrol agents to control ARD.
苹果再植病（ARD）在世界所有主要水果种植区均有报道，通常由生物因素（致病真菌）和非生物因素（酚类化合物）引起。为了阐明根皮苷作用下串珠镰刀菌的蛋白质组差异，并探究串珠镰刀菌作为苹果再植病病原菌的潜在机制，进一步明确了镰刀菌属在苹果再植病中的作用。
本文采用定量蛋白质组学方法iTRAQ分析技术，分析根皮苷处理前后串珠镰刀菌的蛋白质组差异。通过qRT-PCR分析对差异表达蛋白进行验证。
共检测到4535种蛋白质，发现293种蛋白质存在1.2倍以上（P<0.05）的差异。深入数据分析显示，有59种蛋白质存在1.5倍以上（P<0.05）的差异，且大多数蛋白质与qRT-PCR结果一致。差异表达蛋白影响多种细胞过程，尤其是代谢过程。在这些代谢途径中，共鉴定出8条显著富集的KEGG途径，在分生孢子和菌丝体中至少有2种丰度不同的附属蛋白。功能途径分析表明，上调蛋白主要分布在氨基糖、核苷酸糖代谢、糖酵解/糖异生和吞噬体途径中。
本研究首次通过iTRAQ标记和LC-MS/MS进行定量蛋白质组学研究，以鉴定根皮苷条件下串珠镰刀菌中的差异表达蛋白。结果证实，串珠镰刀菌呈现出独特的蛋白质谱，表明该物种对根皮苷环境的适应机制。这些结果加深了我们对串珠镰刀菌响应根皮苷诱导剂的蛋白质组的理解，并为进一步探索提高该真菌作为生物防治剂控制苹果再植病的效率提供了依据。
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