Laboratory of Immunology and Inflammation, Department of Cell Biology, University of Brasilia, Brasília, DF, Brazil.
Central Laboratory of Federal District (LACEN), Brasília, Brazil.
Sci Rep. 2021 Jan 14;11(1):1421. doi: 10.1038/s41598-020-80893-y.
Gut microbiota composition can modulate neuroendocrine function, inflammation, and cellular and immunological responses against different pathogens, including viruses. Zika virus (ZIKV) can infect adult immunocompetent individuals and trigger brain damage and antiviral responses. However, it is not known whether ZIKV infection could impact the gut microbiome from adult immunocompetent mice. Here, we investigated modifications induced by ZIKV infection in the gut microbiome of immunocompetent C57BL/6J mice. Adult C57BL/6J mice were infected with ZIKV and the gut microbiota composition was analyzed by next-generation sequencing of the V4 hypervariable region present in the bacterial 16S rDNA gene. Our data showed that ZIKV infection triggered a significant decrease in the bacteria belonging to Actinobacteria and Firmicutes phyla, and increased Deferribacteres and Spirochaetes phyla components compared to uninfected mice. Interestingly, ZIKV infection triggered a significant increase in the abundance of bacteria from the Spirochaetaceae family in the gut microbiota. Lastly, we demonstrated that modulation of microbiota induced by ZIKV infection may lead to intestinal epithelium damage and intense leukocyte recruitment to the intestinal mucosa. Taken together, our data demonstrate that ZIKV infection can impact the gut microbiota composition and colon tissue homeostasis in adult immunocompetent mice.
肠道微生物群落的组成可以调节神经内分泌功能、炎症以及针对不同病原体（包括病毒）的细胞和免疫反应。寨卡病毒（ZIKV）可以感染成年免疫功能正常的个体，并引发大脑损伤和抗病毒反应。然而，目前尚不清楚寨卡病毒感染是否会影响成年免疫功能正常的小鼠的肠道微生物组。在这里，我们研究了寨卡病毒感染对免疫功能正常的 C57BL/6J 小鼠肠道微生物组的诱导修饰。成年 C57BL/6J 小鼠感染寨卡病毒，通过下一代测序分析肠道微生物组的 V4 高变区，该区域存在于细菌 16S rDNA 基因中。我们的数据显示，寨卡病毒感染导致厚壁菌门和拟杆菌门的细菌数量显著减少，而 Deferribacteres 和螺旋体门的细菌数量增加。有趣的是，寨卡病毒感染导致肠道微生物组中螺旋体科细菌的丰度显著增加。最后，我们证明了寨卡病毒感染引起的微生物群调节可能导致肠道上皮损伤和大量白细胞募集到肠道黏膜。总之，我们的数据表明寨卡病毒感染可以影响成年免疫功能正常的小鼠肠道微生物组的组成和结肠组织的稳态。
